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. 2002 Sep;22(17):6056–6069. doi: 10.1128/MCB.22.17.6056-6069.2002

FIG. 6.

FIG. 6.

Direct binding of Pex7p to Pex13p1-100. (A) Yeast myc-Pex7p specifically interacts with GST-Pex13p1-100. Equal amounts of GST or GST-Pex13p1-100 were loaded onto affinity columns and incubated with total cell lysates of a wild-type strain or the myc-Pex7p-expressing wild-type or pex18Δ pex21Δ strains. After the columns were washed, bound proteins were eluted and analyzed by immunoblotting for the presence of myc-Pex7p (upper panel). The presence of GST and GST-Pex13p1-100 in these eluates was determined by Coomassie blue staining (lower panel). (B) Binding of Pex7p to Pex13p in vitro. Equal amounts of GST or GST-Pex13p1-100 were loaded onto affinity columns and incubated with soluble extracts of bacterially expressed His6-tagged Pex7p (+) or a mock-transformed E. coli strain (−). The retention of His6-Pex7p was analyzed by immunoblotting (upper panel). The lower panel shows the presence of GST and GST-Pex13p1-100 in these eluates.