FIG. 7.
The Pex7p-binding region of Pex13p is important for its function. (A) Expression of a truncated Pex13p variant in pex13Δ cells. Whole-cell extracts of strain UTL-7Apex13Δ that had expressed plasmid-borne copies of either full-length Pex13p (Pex13p1-386) or an N-terminally truncated version of Pex13p (Pex13p56-386) were subjected to immunoblot analysis with antiserum against Pex13p. The asterisk denotes proteins that had cross-reacted with the antiserum. (B) Complementation analysis of pex13Δ with truncated Pex13p56-386. The indicated strains were spotted as a series of 10-fold dilutions on an oleic acid plate and incubated for 5 days at 30°C.