FIG. 4.

Morphology of epididymal sperm from Spag6^−/− mice. (A) Wild-type sperm. (B) Sperm cell from a Spag6^−/− mouse showing a disruption of the midpiece. (C) Headless sperm from a Spag6^−/− mouse. (D) Sperm cell with a truncated flagellum and abnormal midpiece from a Spag6^−/− mouse. (E) Phase-contrast figure of wild-type sperm. (F) Immunostaining of Spag6 in Triton X-100-permeabilized wild-type sperm showing staining along the tail. (G) Phase-contrast image of Spag6-deficient sperm. (H) Absence of Spag6 staining in Spag6-deficient sperm. Arrowheads indicate abnormalities.