TABLE 2.
Motility characteristics of sperm from Spag+/+, Spag+/−, and Spag6−/− micea
| Spag genotype | n | Sperm concn (106/ml) (mean ± SD) | % Motile sperm (mean ± SD) (range of mean) | VCL (mean ± SD) (range of mean) | LIN (mean ± SD) (range of mean) |
|---|---|---|---|---|---|
| +/+ | 3 | 82 ± 31b | 52 ± 6b (46-58) | 341 ± 33b (313-378) | 35 ± 5b, c (30-40) |
| +/− | 3 | 65 ± 35b | 56 ± 5b (52-62) | 252 ± 37c (218-292) | 38 ± 4c (34-42) |
| −/− | 3 | 26 ± 9b | 8 ± 9c (0.3-18) | 136 ± 8d (127-142) | 27 ± 3b (24-30) |
a
Computer-assisted sperm analysis was performed as described in the text. The number of motile sperm samples analyzed for each genotype ranged from 289 to 1,107 for wild-type mice, 425 to 635 for heterozygous mice, and 11 to 366 for nullizygous mice. n = number of males tested; VCL, mean curvilinear velocity; LIN = linearity, the best estimate of the straightness of a sperm cell's track (100 = a straight line). Means with different superscripts (b, c, and d) in the same column are statistically significantly different: P < 0.05 by analysis of variance and Newman-Keuls multiple-comparison test.