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Further fractionation of the hPRC-H complex. (A) Immunoblot of 25 μl (∼5%) of each HiTrap-S fraction visualized with the indicated antibody. For SNF2H and YY1, 250 μl was TCA precipitated prior to analysis. (B) Silver staining of HiTrap-S fractions. Fractions (250 μl) were TCA precipitated and separated by SDS-7.5% PAGE.
