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. 2002 Sep;22(17):6070–6078. doi: 10.1128/MCB.22.17.6070-6078.2002

FIG. 5.

FIG. 5.

hPRC-H activity is specific to polynucleosomal templates. (A) hPRC-H inhibits Swi/Snf without disrupting nucleosome position. End-labeled 5S arrays were treated as described in the legend to Fig. 4B, except that HhaI was not added. Following incubation of the templates with Swi/Snf, increasing amounts of MNase were added to each reaction mixture. Lanes: 1 to 4, control template; 5 to 8, 25 fmol of Swi/Snf; 9 to 12, same as lanes 5 to 8 with ∼12 fmol of hPRC-H; 13 to 16, with ∼12 fmol of dPRC1. All reaction mixtures are from the same experiment; nucleosome positions align when samples are analyzed side by side (data not shown). (B) hPRC-H does not block Swi/Snf remodeling on mononucleosomes. Internally labeled TPT mononucleosomes (1 ng) (47) were incubated as described in the legend to Fig. 4B with 200 ng of Swi/Snf and PstI. Amounts of hPRC-H (M33) and dPRC1 are ∼1.2, 4, and 12 fmol.