FIG. 7.

Overexpression of AA-TCTP leads to an increase in the number of multinucleated cells, and overexpression of wt-TCTP and AA-TCTP leads to an increase in the number of cell deaths and cells with ball-like condensed DNA. (a to c) GFP (a), GFP-wt-TCTP (b), and GFP-AA-TCTP (c) were overexpressed in HEK293 cells. Panel c shows an example of the multinucleate phenotype. (d and e) Overexpression of either form of TCTP leads to an increase in the number of rounded cells with ball-like condensed DNA (d) and the number of cells undergoing cell death (e). DNA is stained with propidium iodide (red) and transfected cells fluoresce green in these images. (f) The microtubule network in these multinucleate HA-tagged AA-TCTP-expressing cells appears interphase-like and is concentrated in the center of the ring of nuclei (upper left). Interphase nontransfected cells possess a microtubule network throughout the cytoplasm (right). The green signal is β-tubulin staining, and the red signal is DNA (propidium iodide staining). (g) A pair of centrosomes also localizes to the center of the nuclear annulus in AA-TCTP-transfected cells, as determined by γ-tubulin staining (green) and DNA staining (red).