FIG. 1.

Targeted disruption of the lpa₂ gene. (A) Genomic map of the wild-type and homologously recombined lpa₂ locus, including exons 1 to 3 (boxes), the targeting construct, and location of the Southern probes (P1/P5 and e2b/e2b′). (B) Southern blot analysis of BamHI-digested genomic tail DNA (10 μg/lane) from mice generated by crossing heterozygotes. The lpa₂ genotypes are shown above. The blot was hybridized with both probes P1/P5 and e2b/e2b′. (C) PCR assay with three primers (lpA2e2mh, lpA2e2m2, and Neo2b) detecting the inheritance of the wild-type lpa₂ allele (249 bp) and mutant lpa₂ allele (207 bp). The relative locations of the three primers are shown above.