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. 2002 Oct;22(19):6921–6929. doi: 10.1128/MCB.22.19.6921-6929.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 8. — LPA-induced stress fiber formation in MEMF cells. (A) Fluorescence microscopy of TRITC-phalloidin-stained wild-type (WT) and lpa₁^(−/−) lpa₂^(−/−) double knockout (double KO) meningeal fibroblast cells, either without stimulation (left) or after 15 min of stimulation with 100 nM LPA (right). (B) Percentages of wild-type (WT), lpa₁^(−/−) single, lpa₂^(−/−) single, and lpa₁^(−/−) lpa₂^(−/−) double knockout meningeal fibroblasts with actin stress fibers after stimulation with increasing concentrations of LPA. (C) Percentages of wild-type (WT) and lpa₁^(−/−) lpa₂^(−/−) double knockout meningeal fibroblasts with actin stress fibers after stimulation with increasing concentrations of S1P. Data shown are the means ± standard errors of triplicate samples. Bar, 10 μm.