FIG. 4.

Mapping the Ttk-binding domain within Phyl. (A) Schematic representation of Phyl proteins and summary of their Ttk-binding activities. The 400-amino-acid Phyl protein can be divided into neutral, basic, and acidic regions based on charge distribution. The Phyl amino acids included in each derivative protein are indicated by numbers. Proteins were labeled with [³⁵S]methionine and assayed for Ttk binding by coimmunoprecipitation with unlabeled Ttk-HA. +, Wild-type binding activity; +/−, reduced binding activity; −, no specific binding activity. (B) Analysis of representative binding reactions between Phyl and Ttk-HA. ³⁵S-labeled Phyl proteins were mixed with Ttk-HA and immunoprecipitated with anti-HA before SDS-PAGE.