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. 2002 Oct;22(19):6759–6766. doi: 10.1128/MCB.22.19.6759-6766.2002

FIG. 2.

FIG. 2.

Mammalian two-hybrid interaction of Dach1 and Eya proteins. (A) One-half microgram of the reporter, pGLMRG5, was cotransfected with or without pfDach1, and pMEya plasmids. pCMV-Flag-2 and pM were used as a control. Luciferase activity in the cell lysate was normalized with β-galactosidase activity of pEFBOSβ-GAL as an internal control. Increasing amounts (0.1 and 0.3 μg) of pMEya1, pMEya2, pMEya3, and pMEya4 were added. The addition of 0.3 μg of pfDach1 is indicated by +. Data reflect luciferase activity relative to that in the presence of pfDach1. (B) GAL4-Dach1 (pMDach1) showed no activation of the reporter. Combinations of 0.5 μg of pMDach1 and 0.5 μg of pHM6Eya3 are shown. Data represent luciferase activity relative to that in the presence of pM and pHM6. Act., activity.