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. 2002 Oct;22(19):6759–6766. doi: 10.1128/MCB.22.19.6759-6766.2002

FIG. 6.

FIG. 6.

Synergy of Six5, Eya3, and Dach1 and effects of E1A on synergy. (A) Interaction with Six, Eya, and Dach was analyzed. Addition of 0.3 μg of pSG424Six5, 0.3 μg of pHM6Eya3, and 0.3 μg of pfDach1 is indicated by +. Relative luciferase activity (Act.) in the presence of pSG424Six5 was set at 1. (B) Effects of E1A and E1A mutations on the transactivation. Shown is the addition of 0.3 μg of pSG424Six5 (columns 2 to 16), 0.3 μg of pHM6Eya3 (columns 7 to 16), and 0.3 μg of pfDach1 (columns 12 to 16) in the presence or absence of 0.01 μg of E1A (columns 3, 4, 8, 9, 13, and 14) or E1A mutation (columns 5, 6, 10, 11, 15, and 16).