SNED1 modulates ECM architecture and cell proliferation via its LDV integrin-binding motif

Abstract

The extracellular matrix (ECM) is a complex scaffold of proteins that supports multicellular structures. Interactions between cells and the ECM via receptors, like integrins, govern cellular phenotypes (e.g., proliferation, adhesion), but also contribute to ECM assembly. Understanding how ECM-receptor interactions regulate matrix assembly is critical to uncover how alterations of the ECM cause or accompany congenital diseases, cancer, or fibrosis.

SNED1 is an understudied ECM protein with roles in development and metastasis. The mechanisms governing its assembly and signaling functions remain largely unknown. SNED1 contains two integrin-binding motifs, RGD and LDV, and we recently showed that its interaction with RGD-integrins mediates cell adhesion. Here, we investigated the role of SNED1/integrin interactions in SNED1 ECM assembly. While SNED1/integrin interactions were not necessary for its initial incorporation in the ECM, interaction with LDV-, but not RGD-, integrins, was required for ECM build-up and the patterning of SNED1 and the fibrillar proteins fibronectin and collagen I. Moreover, SNED1/LDV-integrin interaction promoted ECM alignment, cell alignment, and cell proliferation, processes essential to SNED1-driven neural crest cell migration during craniofacial development and breast cancer invasion.

SUMMARY STATEMENT

The LDV integrin-binding motif in SNED1, but not the RGD integrin-binding motif, mediates ECM remodeling and controls cell alignment and cell proliferation.

INTRODUCTION

The extracellular matrix (ECM) is a complex assembly of proteins and glycans that constitutes the architectural scaffold of multicellular structures (Hynes and Naba, 2012; Karamanos et al., 2021). Beyond physical support, the ECM provides biochemical and biomechanical cues transduced by receptors, such as the integrins, that regulate a wide range of cellular functions, including adhesion, migration, and proliferation (Chastney et al., 2025; Jones and Jones, 2023; Kanchanawong and Calderwood, 2023; Moreno-Layseca and Streuli, 2014; Pally and Naba, 2024; Saraswathibhatla et al., 2023; Yamada et al., 2019). The building of the ECM is a multi-step process that starts with the deposition of fibrillar ECM proteins, like fibronectin and collagen I, in the extracellular space (Kadler et al., 2008; Naba, 2024; Schwarzbauer and DeSimone, 2011). This initial template serves as a platform for the assembly of additional fibrillar and non-fibrillar proteins to form the mature ECM (Naba, 2024). Mechanistically, ECM build-up requires tightly regulated ECM protein-protein interactions, but also interactions between ECM proteins and their receptors, such as the integrins (Mao and Schwarzbauer, 2005; Musiime et al., 2021; Naba, 2024; Sun et al., 2025). Disruption of ECM scaffold integrity has severe consequences, ranging from congenital diseases (Lamandé and Bateman, 2020) to impaired tissue repair, cancer, and fibrosis (Bonnans et al., 2014; Lu et al., 2011). Deciphering the mechanisms underlying homeostatic ECM assembly and remodeling is thus a prerequisite to understanding how the ECM contributes to pathological processes.

We have recently identified the first functions of an understudied fibrillar ECM protein, SNED1 (Sushi, Nidogen, and EGF-like domains 1), and found that it is critical for embryonic development, since Sned1 knockout mice died shortly after birth due to craniofacial malformations (Barqué et al., 2021). We have also found that SNED1 promotes breast cancer metastasis (Naba et al., 2014), a process that, like craniofacial morphogenesis, requires structural support and signals from the ECM scaffold to guide cell adhesion, migration, and proliferation (Gallik et al., 2017; Stanger and Wahl, 2024; Walma and Yamada, 2020). Mechanistically, the SNED1 sequence contains two integrin binding motifs, RGD and LDV, and we have recently shown that SNED1 mediates cell adhesion via RGD integrins, namely α5β1 and αvβ3, but not LDV integrins, such as α4β1 (Pally et al., 2025). However, to date, we do not know how SNED1 assembles into the ECM and whether it exerts other functions beyond mediating cell adhesion.

In this short report, we investigated a possible role for integrins in SNED1 fibrillar ECM assembly. We found that mutating the two integrin-binding motifs, RGD and LDV, in SNED1, alone or in combination, did not impair initial SNED1 deposition. However, we observed that mutation of the LDV motif, but not of the RGD motif, significantly altered overall ECM architecture and build-up. In a companion study submitted with this manuscript by Leverton et al. (Leverton et al., 2026), we observed that SNED1 closely colocalizes with fibronectin and collagen I and requires their presence for initial fibrillar assembly in the ECM. However, we also reported that, as ECM matures in vitro, SNED1 fibrils separate from the fibronectin and the collagen I meshwork. Here, we found that, instead of separating from each other as observed with SNED1^WT, SNED1^LAV fibers remained intertwined within the fibronectin and collagen I meshwork, suggesting a role for SNED1-LDV motif in the maturation of the ECM. Functionally, we found that SNED1^LAV expression perturbed ECM alignment and cell alignment and reduced cell proliferation. Altogether, our results point to a role for the LDV integrin-binding motif in SNED1, independent of the canonical LDV receptors α4 and β1 integrins, in homeostatic ECM remodeling and ECM fiber alignment, cell alignment, and cell proliferation.

RESULTS AND DISCUSSION

Establishment of a cell culture system to test the roles of SNED1 integrin-binding motifs in ECM assembly, patterning, and cellular phenotypes

To explore the possible contribution of integrins in the assembly and maturation of the architecture of the SNED1 fibrillar ECM meshwork, we engineered immortalized embryonic fibroblasts isolated from Sned1 knockout mice (Sned1^KO iMEFs) (Barqué et al., 2021) to stably expressed GFP-tagged constructs of SNED1 in which we mutated its integrin-binding motifs: the RGD motif into RGE and the LDV motif into LAV, alone or in combination. These mutations have been previously shown to disrupt interactions with the eight RGD integrins (namely α5β1, α8β1, the five αv heterodimers, and the platelet-specific αIIbβ3) and some of the “LDV integrins” (namely α4β1, α9β1, α4β7), respectively (Campbell and Humphries, 2011; Cherny et al., 1993; Hautanen et al., 1989; Makarem and Humphries, 1991; Ruoslahti, 1996; Vogelezang et al., 2001). Here, we confirmed that the constructs were expressed by the cells (Supplemental Figure S1A). Of note, we observed that SNED1^RGE-GFP was produced in higher abundance than SNED1^WT-GFP, SNED1^LAV-GFP, or SNED1^RGE/LAV, whose levels are comparable (Supplemental Figure S1B). We also confirmed that the mutant forms of SNED1 could still be secreted, as they were detected in the conditioned culture media (Supplemental Figure S1C). We next tested whether mutated forms of SNED1 were presented into the ECM using the sodium deoxycholate (DOC) solubility assay. We found that, in addition to being detected in the DOC-soluble protein fraction (Supplemental Figure S1D and S1E), which is enriched for intracellular proteins, all proteins were detected in the DOC-insoluble protein fraction, enriched for ECM proteins, as early as 48 hours post-seeding (Supplemental Figure S1F). This indicates that disrupting the putative integrin-binding motifs in SNED1 does not impair its initial incorporation into the insoluble ECM.

Disruption of the RGD and LDV integrin-binding motifs in SNED1 does not impair the early step of its ECM assembly

Using this experimental cell system, we first examined whether mutations in the integrin-binding sites of SNED1 affected its incorporation into the ECM scaffold. Using confocal immunofluorescence microscopy, we monitored the assembly and organization of SNED1 and two major fibrillar ECM proteins, fibronectin and collagen I, in the ECM produced by cells for 3 days in culture. We found that neither the disruption of the RGD motif nor the disruption of the LDV motif altered the ability of SNED1 to incorporate in the ECM (Figure 1A and 1B). We also found that expression of the mutant forms of SNED1 did not alter the patterning of fibronectin (Figure 1A) or collagen I (Supplemental Figure S2A). Expression of integrin-binding mutant forms of SNED1 did not statistically significantly alter the colocalization of SNED1 with fibronectin (Figures 1C and 1D; p = 0.0597) or with collagen I (Supplemental Figures S2B–S2D) that we reported in the companion study by Leverton et al (Leverton et al., 2026). Of note, the disruption of both putative integrin-binding sites together (SNED1^RGE/LAV) did not alter the initial fibrillar assembly of SNED1 in the ECM either (Supplemental Figures S2E and S2F).

Figure 1. Disruption of the RGD or LDV integrin-binding motifs in SNED1 does not affect the early stages of ECM assembly or patterning of SNED1.

A. XY orthogonal maximum projections show SNED1 (cyan) and fibronectin (magenta) fibers in ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panels), SNED1^RGE-GFP (middle panels), or SNED1^LAV-GFP (bottom panels) and decellularized 3 days post-seeding. Merge panels show overlap between SNED1 and fibronectin signals. Scale bar: 30 μm. Images are representative of three independent biological replicates.

B. XZ orthogonal maximum projections show fibronectin (magenta) and SNED1-GFP (cyan) signal in the ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel), SNED1^RGE-GFP (middle panel), or SNED1^LAV-GFP (right panel), decellularized 3 days post cell-seeding. Images are representative of three independent biological replicates.

C. Representative line graphs show Manders overlap coefficient between SNED1 and fibronectin for each Z-slice where in-focus signal was detected in the ECM produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panel), SNED1^RGE-GFP (middle panel) or SNED1^LAV-GFP (bottom panel) and decellularized 3 days post-seeding.

D. Dot plots depict the overlap between the SNED1 and fibronectin (FN) signals in ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel), SNED1^RGE-GFP (middle panel) or SNED1^LAV-GFP (right panel) and decellularized 3 days post-seeding. Lower and higher data points represent the beginning and end of in-focus signal detected in the ECM. Data points are normalized to the Z-slice value where SNED1’s signal is detected. Data is represented as mean ± standard deviation. Unpaired t-test with Welch’s correction was performed to test statistical significance. *p<0.05. Note that the plot depicting the SNED1^WT-GFP condition corresponds to the data presented in the companion manuscript by Leverton et al.

E. XY orthogonal maximum projections show fibril assembly of exogenously added fibronectin (red) by Sned1^KO iMEFs overexpressing GFP alone (nucleus staining; grey). Merge panels show cells and fibronectin fibrils signals. Images are representative of two independent biological replicates. Scale bar: 30 μm.

F. XY orthogonal maximum projections of Sned1^KO iMEFs overexpressing GFP alone (nucleus staining; grey) incubated in the presence of purified SNED1^WT-FLAG (orange). Images are representative of two independent biological replicates. Scale bar: 30 μm.

To further test whether cells are required for the early step of SNED1 assembly, we asked whether cells were capable of assembling SNED1 fibers if incubated in presence of exogenous SNED1. This approach has been used to demonstrate that other ECM proteins such as fibronectin and fibrillin 1 required cell-binding via integrins for their fibrillar assembly (Sabatier et al., 2009; Sechler et al., 1997). To do so, we supplemented the culture medium of Sned1^KO iMEFs overexpressing GFP alone with exogenous fibronectin, a protein known to require interaction with integrins to initiate its fibrillar assembly, or SNED1^WT-FLAG. After 3 days in culture, unlike fibronectin (Figure 1E), we did not observe SNED1 fibrils (Figure 1F).

Together with the observations reported in the companion paper (Leverton et al., 2026), our findings demonstrate that the initial step of SNED1 incorporation into the ECM is integrin-independent and instead relies on the presence of fibrillar ECM proteins, fibronectin and collagen I. Interestingly, this represents a distinct mechanism from integrin-mediated initial ECM assembly of other ECM proteins, such as that of fibronectin, where fibronectin fibril assembly is initiated by the interaction of α5β1 integrins with its RDG motif before involving ECM protein-ECM protein interactions (Mao and Schwarzbauer, 2005; Singh et al., 2010).

Disruption of the LDV but not the RGD integrin-binding motif in SNED1 impairs ECM remodeling and SNED1 distribution in mature ECMs

As discussed in the companion paper to this study (Leverton et al., 2026), we found that after early assembly, ECM produced by immortalized mouse embryonic fibroblasts undergoes remodeling and that the meshwork of SNED1^WT fibers, which initially overlaps with fibronectin and collagen I (Figures 1A–1D), separates from the meshwork containing collagen I and fibronectin (Figures 2A–2D).

Figure 2. Disruption of the LDV but not the RGD integrin-binding motif in SNED1 impairs ECM build-up, remodeling, and alignment.

A. XY orthogonal maximum projections show SNED1 (cyan) and fibronectin (magenta) fibers in the ECM produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panels), SNED1^RGE-GFP (middle panels), or SNED1^LAV-GFP (bottom panels) and decellularized 9 days post-seeding. Merge panels show overlap between SNED1 and fibronectin signals. Scale bar: 30 μm. Images are representative of three independent biological replicates.

B. XZ orthogonal maximum projections show fibronectin (magenta) and SNED1-GFP (cyan) signals in the ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel), SNED1^RGE-GFP (middle panel), or SNED1^LAV-GFP (right panel) decellularized at 9 days post cell-seeding. Images are representative of three independent biological replicates.

C. Representative line graphs show Manders overlap coefficient between SNED1 and fibronectin for each Z-slice where in-focus signal was detected in the ECM produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panel), SNED1^RGE-GFP (middle panel), or SNED1^LAV-GFP (bottom panel), decellularized 9 days post-seeding.

D. Dot plots depict the overlap between SNED1 and fibronectin (FN) signals in ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel), SNED1^RGE-GFP (middle panel), or SNED1^LAV-GFP (right panel) decellularized at 9 days post-seeding. Lower and higher data points represent the beginning and end of in-focus signal detected in the ECM. Data points are normalized to the Z-slice value where SNED1’s signal is detected. Data is represented as mean ± standard deviation. Unpaired t-test with Welch’s correction was performed to test statistical significance. *p<0.05, ***p<0.001, ****p<0.0001.Note that the plot depicting the SNED1^WT-GFP condition corresponds to the data presented in the companion manuscript by Leverton et al.

E. Dot plot depicts the overall thickness of ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP, SNED1^RGE-GFP, or SNED1^LAV-GFP decellularized at 9 days post-seeding. Data is represented as mean ± standard deviation from three biological replicates, with at least 4 imaged fields per replicate and timepoint. Unpaired t-test with Welch’s correction was performed to test statistical significance. ****p<0.0001.

F. Dot plot depicts SNED1 thickness in the ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP or SNED1^LAV-GFP decellularized at 9 days post-seeding. Data is represented as mean ± standard deviation from three biological replicates, with at least 4 imaged fields per replicate and timepoint. Unpaired t-test with Welch’s correction was performed to test statistical significance. ****p<0.0001.Note that the plot depicting the SNED1^WT-GFP condition corresponds to the data presented in the companion manuscript by Leverton et al.

G. Histogram depicts SNED1 fiber alignment in ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red). Data is represented as the mean ± standard deviation from three independent biological replicates with at least four non-overlapping images from each biological replicate.

H. Histogram depicts fibronectin fiber alignment in ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red). Data is represented as the mean ± standard deviation from three independent biological replicates with at least four non-overlapping images from each replicate.

I. Dot plot shows area fraction (density) of SNED1 fibers in decellularized ECMs produced by Sned1^KO iMEFs overexpressing either SNED1^WT-GFP (black) or SNED1^LAV-GFP (red). Data is represented as mean ± standard deviation from three biological replicates, with at least 4 imaged fields per replicate. Unpaired t-test with Welch’s correction was performed to test statistical significance. ****p<0.0001. Note that the plot depicting the SNED1^WT-GFP condition corresponds to the data presented in the companion manuscript by Leverton et al.

J. Dot plot shows area fraction (density) of fibronectin signal in decellularized ECMs produced by Sned1^KO iMEFs overexpressing either SNED1^WT-GFP (black) or SNED1^LAV-GFP (red). Data is represented as mean ± standard deviation from three biological replicates, with at least 4 imaged fields per biological replicate. Unpaired t-test with Welch’s correction was performed to test statistical significance (ns).

Similarly to what we observed upon overexpression of SNED1^WT, we observed that, in mature ECMs, the meshwork of SNED1^RGE fibers separates from the fibronectin (Figures 2A–2D) and collagen meshworks (Supplemental Figures S3A–S3D). Similarly to SNED1^WT, we observed a decrease in the colocalization between the SNED1^RGE-GFP and fibronectin (Figures 2A, 2B, 2C) or collagen I (Supplemental Figures S3A, S3B, S3C). Assessment of the distribution of the proteins through the thickness of the ECM using XZ orthogonal maximum intensity projection revealed that in ECMs produced by cells expressing SNED1^WT or SNED1^RGE, the most basal portion of the ECM is devoid of fibronectin; conversely the upper portion of the ECM (~1.5 μm) exclusively contains fibronectin and collagen I and is devoid of SNED1 (Figures 2B and 2D and Supplemental Figures S3C and S3D, respectively).

In stark contrast, ECM remodeling and partitioning of different ECM layers did not occur upon expression of SNED1^LAV. The quantitative analysis of the spatial overlap, in the XY (Figure 2C) and in the XZ (Figure 2D) dimensions, showed that SNED1^LAV and fibronectin colocalized and occupied the same portion of the ECM (Figures 2C and 2D, respectively), although the fibronectin extended a bit further (~0.7 μm) than the SNED1 staining apically upper mean of fibronectin slightly but statistically significantly shifted upward (Figure 2D). Similar observations were made with collagen I (Supplemental Figures S3A–S3D). In addition, we observed that the expression of SNED1^LAV prevented ECM build-up and remodeling and resulted in overall thinner ECM (~3.8 μm; Figure 2E) and thinner SNED1 layer (~2.9 μm; Figure 2F), suggesting that this mutant acts as a dominant negative protein on ECM remodeling and maturation. Of note, the combined disruption of the two integrin-binding sites in SNED1 (SNED1^RGE/LAV) phenocopied the SNED1^LAV phenotype (Supplemental Figures S3E and S3F).

Mutation of the LDV integrin-binding motif in SNED1 affects fiber alignment

A closer examination of the pattern of distribution of SNED1 in a mature (9-day old) ECM revealed that, in contrast to highly aligned SNED1^WT, SNED1^LAV fibers are more disorganized and do not follow a specific alignment pattern (Figure 2A). Quantification of SNED1 fiber orientation showed that SNED1^WT fibers displayed a bimodal distribution with two distinct peaks, while SNED1^LAV fibers showed a wider distribution of angles with no distinct peaks (Figure 2G). We observed that the mutation of the LDV integrin-binding motif in SNED1 also impaired the alignment of fibronectin fibers (Figure 2H). In addition, the density of SNED1-positive staining was significantly reduced in ECM produced by cells expressing SNED1^LAV as compared to the ECM produced by cells expressing SNED1^WT (Figure 2I). Of note, these changes cannot be attributed to differential protein abundance, since SNED1^WT and SNED1^LAV are produced in the same proportion by cells (Supplemental Figure S1A). Importantly, the density of fibronectin-positive staining was not altered in ECM produced by cells expressing SNED1^LAV in comparison to the ECM produced by cells expressing SNED1^WT (Figure 2J). Altogether, our observations demonstrate a role for SNED1’s LDV motif not only in the patterning of SNED1, but also the modulation of overall ECM build-up and fibrillar patterning.

SNED1 fibrillar rearrangement does not require canonical LDV integrins

In light of our previous findings that the RGD but not the LDV motif in SNED1 mediates cell adhesion (Pally et al., 2025), we propose that SNED1-integrin interaction sites exert different roles. Specifically, our results point to a possible role for the LDV motif of SNED1, and hence its possible interaction with LDV integrins in remodeling the ECM meshwork and establishing the partitioning of different ECM layers. Interestingly, Sechler et al. have previously found that, in the absence of an RGD motif, fibronectin fibril formation can still occur but through an alternative mechanism engaging α4β1 integrin interaction with fibronectin’s LDV motif (Sechler et al., 2000). However, in SNED1, the function of the LDV motif in ECM remodeling does not appear to be redundant with that of the RGD motif. We thus next sought to test the possible involvement of LDV integrins in SNED1 fibrillar rearrangement. While we detected the presence of α4, α5, α9, and β1 integrins in overall similar proportion at the membrane of mouse embryonic fibroblasts expressing the different mutant forms of SNED1 (Supplemental Figure S4A), we found that mouse embryonic fibroblasts could not adhere to a well-characterized LDV-containing ligand and known ligand of α4β1, α4β7, and α9β1 integrins, VCAM-1 (Supplemental Figure S4B) (Clements et al., 1994; LaFoya et al., 2018), indicating that while present, these receptors may not be active at the surface of MEFs. In agreement with these findings, we found that treating SNED1-GFP-expressing MEFs with a functional blocking antibody directed against β1 integrin after the initial ECM assembly phase, did not phenocopy the effect observed upon expression of SNED1^LAV-GFP (Supplemental Figures S4C to S4E).

Altogether, our data suggest that the phenotype observed upon mutation of the LDV motif in SNED1 is independent of canonical integrins known to date to engage with the LDV motif. We can speculate that the LDV motif in SNED1 may engage with different molecular partners whose identification will be the focus of future studies.

ECM containing SNED1^LAV alters cell alignment

We next sought to determine the possible functional consequence of the altered ECM phenotype (i.e., thinner ECM, retained colocalization between SNED1, fibronectin, and collagen I, and impaired ECM fiber alignment) observed upon expression of SNED1^LAV. Since one of the main functions of the ECM is to serve as a substrate for cell adhesion, we first tested whether cells could adhere to SNED1^LAV-containing ECMs^. We found that MEFs adhered to the same extent to decellularized ECM produced by cells overexpressing SNED1^WT than to the ECM produced by cells overexpressing SNED1^LAV (Figure 3A). We also observed that cells seeded on ECMs containing SNED1^LAV tended to spread less than those seeded on ECMs containing SNED1^WT (Figure 3B). However, the quantification of morphometric parameters, such as cell area, and aspect ratio, did not meet statistical significance (Figure 3C and 3D).

Figure 3. Mutation of the LDV motif in SNED1 impairs cell alignment.

A. Left panels: Microscopy images show cells (red) that have adhered after 30 min to ECMs produced by cells overexpressing SNED1^WT-GFP (left panel) or SNED1^LAV-GFP (right panel) and decellularized at 9 days post-seeding. Images representative of three biological replicates. Scale bar: 200 μm. Right panel: Dot plot depicts the number of adherent cells per field on decellularized ECM containing SNED1^WT (black) or SNED1^LAV (red). Data is represented as mean ± standard deviation from three biological replicates, with at least 10 fields imaged per coverslip and at least two coverslips per replicate. Unpaired t-test with Welch’s correction did not show any statistical significance.

B. Microscopy images show the spreading of Sned1^KO iMEFs overexpressing GFP alone (visualized through phalloidin-AF647 staining) seeded on decellularized ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel) or SNED1^LAV-GFP (right panel) for 9 days. Images are representative of three biological replicates. Scale bar: 50 μm.

C. Dot plot depicts the area of Sned1^KO iMEFs overexpressing GFP alone seeded on decellularized ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red) for 9 days. Data is represented as mean ± standard deviation from three biological replicates, with at least 10 imaging fields per coverslip, and at least two coverslips per replicate. Unpaired t-test with Welch’s correction did not show any statistical significance.

D. Dot plot depicts the aspect ratio of Sned1^KO iMEFs overexpressing GFP alone seeded on decellularized ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red) for 9 days. Data is represented as mean ± standard deviation from three biological replicates, with at least 10 imaging fields per coverslip, and at least two coverslips per replicate. Unpaired t-test with Welch’s correction did not show any statistical significance.E. XY orthogonal maximum intensity projections show F-actin staining (red) in Sned1^KO iMEFs overexpressing SNED1^WT-GFP (left panel) or SNED1^LAV-GFP (right panel) at 9 days post seeding. Scale bar: 50 μm. Images are representative of three independent biological replicates.

F. Histogram depicts F-actin alignment in Sned1^KO iMEFs overexpressing SNED1^WT-GFP or SNED1^LAV-GFP at 9 days post seeding. Data is represented as the mean ± standard deviation from three independent biological replicates with at least four non-overlapping images from each replicate.

G. XY orthogonal maximum intensity projections show F-actin staining (red) of Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panel) or SNED1^LAV-GFP (bottom panel) when cultured for 2 days on ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP or SNED1^LAV-GFP and decellularized at 9 days post-seeding. Images are representative of three independent biological replicates. Scale bar: 50 μm.

H. Histogram show F-actin alignment in Sned1^KO iMEFs overexpressing SNED1^WT-GFP (top panel) or SNED1^LAV-GFP (bottom panel) when cultured on decellularized ECMs containing SNED1^WT-GFP or SNED1^LAV-GFP. Data is represented as mean ± standard deviation from three independent biological replicates with at least four non-overlapping images from each replicate.

Since we showed that mutation of the SNED1 LDV motif altered ECM alignment and patterning, we next asked whether cells followed the same pattern. We thus quantified the orientation of actin filaments in cells expressing SNED1^WT or SNED1^LAV after 9 days in culture and found that cells expressing SNED1^LAV were much more disorganized than cells expressing SNED1^WT (Figures 3E and 3F). This phenotype mirrors the data reported in Figure 2 on SNED1 and fibronectin fiber alignment patterns.

To determine whether ECM architecture can dictate cell alignment, we performed a cross-seeding experiment whereby we cultured cells overexpressing SNED1^WT or SNED1^LAV on decellularized ECM produced by cells expressing either SNED1^WT or SNED1^LAV for 48 hours. Interestingly, cells overexpressing SNED1^WT followed the disorganized alignment patterns of a SNED1^LAV-containing ECM (Figure 3G, top right panel), as quantified in Figure 3H Vice-versa, cells overexpressing SNED1^LAV were able to align when seeded on a SNED1^WT-containing ECM (Figure 3G, bottom left panel), as quantified in Figure 3H. These results demonstrate that ECM architecture is sufficient to govern cell alignment and supersedes gene expression.

Abrogation of the LDV integrin-binding motif in SNED1 decreases cell proliferation

In addition to mediating cell adhesion and organization, the ECM transmits biochemical and mechanical signals to cells and initiate molecular cascades that govern various cellular processes, including cell proliferation (Jones and Jones, 2023). During the course of this study, we observed that cells expressing SNED1^LAV proliferated more slowly than cells expressing SNED1^WT (Figure 4A). We thus asked whether SNED1^WT or SNED1^LAV-containing ECMs altered cell proliferation. To do so, we generated ECMs from SNED1^WT- or SNED1^LAV-expressing cells and decellularized them 9 days post-seeding. We then seeded Sned1^KO iMEFs on these decellularized ECMs. We used this cellular system to avoid interference from Sned1 expression by re-seeded cells. 4 days post-reseeding, we found that cells seeded on a SNED1^LAV-containing ECM had proliferated significantly less compared to cells seeded on a SNED1^WT-containing ECM (Figure 4B), suggesting that SNED1 acts in a non-cell autonomous manner.

Figure 4. Mutation of the LDV motif in SNED1 impairs cell proliferation.

A. Line graph shows proliferation of Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red) at 2 and 4 days post-seeding. Data is represented as mean ± standard deviation from three biological replicates. Unpaired t-test with Welch’s correction was performed to test statistical significance. *p<0.05.

B. Dot plot shows proliferation of Sned1^KO iMEFs overexpressing GFP at 4 days when cultured on ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP (black) or SNED1^LAV-GFP (red) and decellularized at 6 days post-seeding. Data is represented as mean ± standard deviation from three biological replicates. Unpaired t-test with Welch’s correction was performed to test statistical significance. *p<0.05.

ECM organization and stiffness have been shown to govern cell proliferation via the mechanosensitive YAP-TAZ pathway (Schwartz and Assoian, 2001; Sun et al., 2016; Totaro et al., 2018). Since we have shown that the expression of SNED1^LAV had a profound impact on the organization of the entire ECM meshwork, we postulate that altered ECM patterning and possible alterations on the mechanical properties of ECMs containing SNED1^LAV, are responsible for the decrease cell proliferation rate. It would thus be interesting in the future to determine the impact of SNED1^LAV on the mechanical properties of the ECM and delineate more precisely which SNED1-dependent, but integrin-independent, signaling pathways are at play.

CONCLUSION

The data presented here provide the first evidence for a role of the LDV integrin-binding motif in SNED1 in the assembly and organization of SNED1 fibrils. We also uncovered a role for SNED1 in the overall organization of the ECM meshwork, including of the meshwork composed of the two most abundant fibrillar ECM proteins, fibronectin and collagen I and in cell phenotypes, cell alignment and proliferation, both key to the pathophysiological processes we have previously incriminated SNED1 in, namely the migration of neural crest cells during developmental craniofacial morphogenesis (Barqué et al., 2021) and breast cancer metastasis (Naba et al., 2014). However, our observations also suggest that the phenotypes observed upon disruption of the LDV motif in SNED1 are independent of the canonical LDV integrins. This raises the possibility that other receptors or other ECM proteins could interact with SNED1 via its LDV motif. The identification of these putative partners and their potential roles in mediating the phenotype observed upon disruption of the LDV motif in SNED1 will be the focus of future investigations and will be critical to determine to what extent SNED1-dependent ECM remodeling contribute to the roles of SNED1 in-vivo.

MATERIALS AND METHODS

Plasmid constructs

The cDNA encoding full-length human SNED1 cloned into pCMV-XL5 was obtained from Origene (clone SC315884). Using a protocol previously described (Pally et al., 2025), we performed site-directed mutagenesis (Agilent, #200519) to introduce mutations to disrupt the two integrin binding sites RGD and LDV, alone (c.C120A; p.D40E; c.A932C; p.D311A) or in combination (c.C120A; p.D40E/c.A932C; p.D311A), in SNED1.

These constructs were then amplified by PCR to insert a FLAG tag at the C-terminus of SNED1 and the BglII and HpaI restriction sites to insert the constructs into the bicistronic retroviral vector pMSCV-IRES-Hygromycin. SNED1^WT-FLAG was used for experiment testing the ability of cells to assemble exogenous SNED1, while SNED1LAV-FLAG was used to determine the ability of SNED1 to regulate cell proliferation.

The cloning of 6X-His-tagged versions of wild-type and mutant forms of SNED1 was previously described (Pally et al., 2025). His-tagged forms of SNED1 were used to coat cell culture dishes and determine the ability of SNED1 to mediate cell adhesion.

The cloning of GFP-tagged SNED1 (hereafter referred to as SNED1^WT-GFP) was previously described (Vallet et al., 2021). Using site-directed mutagenesis (Agilent, #200519), we generated the following constructs: SNED1^RGE-GFP (c.C120A; p.D40E), SNED1^LAV-GFP (c.A932C; p.D311A), and SNED1^RGE/LAV-GFP (c.C120A; p.D40E/c.A932C; p.D311A). These constructs were then shuttled into the bicistronic retroviral vector pMSCV-IRES-Puromycin between the BglII and EcoRI sites, as previously described (Vallet et al., 2021) and were used for all imaging experiments.

The sequences of the primers used for site-directed mutagenesis and subcloning are listed in Supplemental Table S1. All constructs were verified by Sanger sequencing at the UIC Genome Research Core facility. Constructs can be obtained upon email request to Dr. Naba (anaba@uic.edu).

Cell culture

Cell maintenance

Immortalized mouse embryonic fibroblasts isolated from Sned1^KO mice (refer to as Sned1^KO iMEFs in the manuscript) (Barqué et al., 2021) stably overexpressing GFP-tagged wild type or integrin-binding mutant forms of SNED1 and human embryonic kidney 293T cells (further termed 293T) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM; Corning, #10-017-CV) supplemented with 10% fetal bovine serum (FBS; Sigma, #F0926) and 2 mM glutamine (Corning, #25-005-CI), this medium will be further referred to as complete medium. U937 cells were kindly gifted by Dr. Sandra Pinho at University of Illinois Chicago and were maintained in RPMI 1640 + 10% FBS + 1X penicillin-streptomycin (Gibco, #15140122). All cell lines were maintained at 37 °C in a 5% CO₂ humidified incubator. All cell lines used in this study were tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (Sigma, #MP0035) in April 2026.

Retrovirus production

293T cells were seeded in a 6-well plate at ~30% confluency. The following day, cells were transfected using Lipofectamine 3000 (Invitrogen, #L3000008) with a mixture containing 1 μg of retroviral vector with the construct of interest and 0.5 μg each of a packaging vector (pCL-Gag/Pol) and vector encoding VSVG coat protein prepared in Opti-MEM (Gibco, #31985070). After 24 hours, the transfection mixture was replaced with fresh complete medium, and cells were cultured for an additional 24 hours. The conditioned medium containing viral particles was collected, filtered through a 0.45 μm filter, and either used immediately for cell transduction or stored at −80 °C for future use.

Generation of cells stably expressing different point mutants of SNED1

Sned1^KO iMEFs were seeded at ∼30% confluency in a 6-well plate. The following day, cells were transduced with undiluted viral particles-containing conditioned medium. 24 hours after transduction, the medium was replaced with fresh complete medium, and cells were allowed to grow for an additional 24 hours before selection with puromycin (5 μg/ml). Once stable cell lines were established, the production and secretion of GFP-tagged mutant forms of SNED1 were confirmed using immunoblotting of total protein extracts (TE) and conditioned medium (CM), respectively, with an anti-SNED1 antibody; see Results. See Supplemental Table S2 for details on antibodies used.

Preparation of fibronectin-depleted FBS

Fibronectin was depleted from FBS using a gelatin Sepharose 4B affinity resin (Cytiva, #17095601), as previously described (Sabatier et al., 2013; Sechler et al., 1996). All steps were performed on ice or in a cold room using ice-cold reagents. Gelatin Sepharose resin was supplied in 20% ethanol and was washed with 1X PBS before packing. 100 mL resin was packed into an Econo column using an Econo gradient pump (Biorad) with a maximum flow rate of 6.0 mL/min. Once packed, the column was equilibrated with 5 column volumes of 1X PBS at a flow rate of 4.5 mL/min. Following equilibration, 300 mL of FBS was loaded onto the column at a flow rate of 4.0 mL/min using an Econo pump and looped for 200 minutes. The flow-through was sterilized using a 0.2μm filter. Fibronectin-depleted FBS was used in the experiments to test the ability to assemble exogenous fibronectin or SNED1 into fibrillar structures.

Protein purification

Conditioned medium collection

Conditioned medium containing recombinant SNED1 (WT or integrin-binding mutants) was collected as described previously (Pally et al., 2025). In brief, 293T cells stably expressing the protein of interest were seeded in a 15-cm dish in complete medium and allowed to grow until they reached 100% confluency. The culture medium was aspirated, and the cells were rinsed with 1X D-PBS containing Ca²⁺ and Mg²⁺ (D-PBS⁺⁺, Cytiva, #SH30264.01). The medium was replaced with serum-free DMEM supplemented with 2 mM glutamine. Serum-free conditioned medium (SFCM) containing the secreted protein of interest was harvested after 48 hours, and cells were allowed to recover for 48 h in complete medium before repeating the next cycle. Cells were discarded after four to five cycles. At each collection time point, an EDTA-free protease inhibitor cocktail (0.067X final concentration; Thermo Fisher Scientific, #A32955) was added to the CM, and the CM was centrifuged at 2,576 × g for 10 minutes at 4 °C to remove any cells or cellular debris. Pre-cleared supernatants were collected and stored at −80 °C until further processing.

Purification of His-tagged and FLAG-tagged SNED1 proteins

Metal affinity protein purification of SNED1^WT-His, SNED1^RGE-His, SNED1^LAV-His, and SNED1^RGE/LAV-His was performed at the UIC Biophysics Core facility as previously described (Pally et al., 2025). Immunoprecipitation of FLAG-tagged forms of SNED1 (SNED1^WT-FLAG, SNED1^RGE-FLAG, SNED1^LAV-FLAG, and SNED1^RGE/LAV-FLAG) was performed in-house using an anti-FLAG resin (Sigma, #A220), as previously described (Pally et al., 2025).

Immunoprecipitation of SNED1-GFP from conditioned medium

iMEFs overexpressing SNED1^WT-GFP, SNED1^RGE-GFP, SNED1^LAV-GFP, or SNED1^RGE/LAV-GFP were seeded at 5.5 × 10⁵ in a sterile tissue-culture-treated 6-well plate. When cells had reached 100% confluency (~48h post-seeding), the culture medium was replaced and supplemented with 50 μg/mL ascorbic acid (Thermo Scientific, #352685000). The conditional medium (CM) was collected 4 days post-seeding and subjected to immunoprecipitation using protein A/G agarose beads (Thermo Scientific, #20421) and either IgG isotype as a negative control (Invitrogen, #PI31903) or an anti-GFP antibody (Sigma, #G6539; Supplemental Table S2). A second negative control, omitting antibodies, was also included. Samples were incubated on a rotating wheel at 4 °C, overnight. Samples were then centrifuged at 8,200 × g at 4 °C for 30 sec. to pellet the beads and immunoprecipitated proteins. Beads were washed three times in cold 1X PBS. Following the final centrifugation, beads were dried and immunoprecipitated proteins were resuspended in 3X Laemmli buffer containing 100 mM DTT. Samples were boiled for 10 minutes at 95 °C and then analyzed via SDS-PAGE and western blot.

Deoxycholate (DOC) solubility assay

The DOC solubility assay was performed as previously described (Pankov and Yamada, 2004; Wierzbicka-Patynowski et al., 2004). iMEFs overexpressing either wild type or integrin-binding mutants of SNED1-GFP were seeded at 5.5 × 10⁵ in 6-well sterile cell culture-treated plates. Approximately 48 hours after seeding, 50 μg/mL ascorbic acid was added to each well. Every 48 hours following that, half of the medium was replaced with complete medium containing 100 μg/mL ascorbic acid. After a specified time in culture (3 days, 6 days, or 9 days), the conditioned medium (CM), containing proteins secreted by the cells, was collected and centrifuged for 3 minutes at 800 × g to remove any cellular debris, and the DOC solubility assay was performed on the cell culture layer composed of cells and their ECM. In brief, cells were washed with cold 1X PBS. 1 mL of DOC lysis buffer (5% deoxycholate, 3M Tris-HCl pH 8.8, 500 mM EDTA), protease inhibitors with EDTA (Thermo Scientific, #A32953), 167 μg/mL DNase I (Sigma, #AMPD1-1KT) was added to each well and incubated at 4°C for 1 hour. Samples were then collected using a sterile scraper and transferred to clean 1.5 mL tubes. Samples were sheared using a 26G needle to reduce viscosity and then centrifuged for 15 minutes at 21,100 × g at 4 °C. The supernatant (DOC-soluble material) was collected in a clean tube and stored at −80 °C. The pellet, containing DOC-insoluble proteins, was stored at −80 °C. Protein quantification of the DOC-soluble fraction was performed via BCA assay (Thermo Scientific, #23225), and samples were prepared for SDS-PAGE.

In parallel, total protein extracts (TE) from cells seeded and treated under the same conditions were collected by lysing the cells in 3X Laemmli buffer (0.1875 M Tris-HCl, 6% SDS, 30% glycerol) containing 100 mM dithiothreitol. Samples were then sheared using a 26G needle to reduce viscosity and boiled for 10 minutes at 95 °C and stored at −80 °C.

Preparation of membrane fraction from cells

Membrane fraction from Sned1^KO iMEFs overexpressing SNED1^WT-GFP, SNED1^RGE-GFP, SNED1^LAV-GFP or SNED1^RGE/LAV-GFP was isolated using subcellular protein fractionation kit as per the manufacturer’s protocol (Thermo, #78840). Briefly, complete medium from cells was aspirated and rinsed with ice cold 1X PBS. Cells were scraped in 1 mL ice cold 1X PBS and collected into a 1.5 mL tube. Cells were pelleted by centrifuging at 500 × g for 3 min at 4 °C and supernatant was aspirated. The packed cell volume was measured and accordingly the cell pellet was resuspended in appropriate volume of ice-cold cytoplasmic extraction buffer (CEB) containing 1X protease inhibitor. Cells were incubated at 4 °C for 10 minutes with gentle mixing. Centrifuge at 500 × g for 5 minutes at 4 °C and immediately transfer the supernatant into a pre-chilled tube. Add appropriate volume of ice-cold membrane extraction buffer (MEB) containing 1X protease inhibitor, vortex for 5 seconds on highest setting and incubate at 4 °C for 10 min with gentle mixing. Centrifuge at 3000 × g for 5 minutes and immediately transfer the supernatant into a prechilled tube. Store the pellet at −80 °C for further processing. Protein concentration in membrane extract was measured using Pierce^™ BCA protein assay kit (Thermo, #23225) as per the manufacturer’s protocol and 20 μg protein was loaded onto SDS-PAGE and probed for α4, α5, α9, β1 integrins and transferrin receptor.

SDS-PAGE and western blot

Protein samples were separated by SDS-PAGE at constant current (20 mA for stacking gel, 25 mA for resolving). Proteins were then transferred to nitrocellulose membranes at a constant current of 100V for 3 hours at 4 °C. Membranes were stained with Ponceau stain for 10 minutes and imaged to confirm the transfer of proteins. Membranes were then blocked in 5% non-fat milk in PBS containing 0.1% Tween-20 (PBST) for 60 minutes at room temperature under constant shaking. Membranes were then incubated in the presence of primary antibodies (Supplemental Table S2) in 5% non-fat milk in PBST overnight with constant shaking at 4 °C. The following day, membranes were washed three times with PBST, followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Supplemental Table S2) in 5% non-fat milk in PBST for 60 minutes with constant shaking at room temperature. Membranes were again washed three times with PBST. The presence of proteins of interest was detected by chemiluminescence, using the Pierce ECL Western blotting substrate (Thermo Scientific #32109). Imaging was performed using a ChemiDoc MP imaging system (Bio-Rad). Images of full, uncropped immunoblots are provided in Supplemental Figure S5.

Quantification of immunoblots

Immunoblots were quantified using Image Lab software (Bio-Rad). The .scn files were opened, and lanes were assigned manually. Protein bands of interest were selected manually, and a background subtraction of 10.0 was applied to all the lanes. The adjusted intensity volume of bands was obtained using the “Analysis table” tab, and data was exported as an Excel file. Protein intensity was normalized to a loading control as described in the figure legends and normalized intensity values were plotted using Prism.

Generation of cell-derived ECMs

Cell seeding

Cell-derived ECMs from iMEFs overexpressing GFP alone or GFP-tagged forms of SNED1 were prepared under aseptic conditions following a protocol previously established by the laboratory of Dr. Jean Schwarzbauer (Harris et al., 2018) with minor modifications. Briefly, glass coverslips were inserted in non-cell-culture-treated sterile plastic plates and coated with autoclaved and sterile-filtered 0.2% gelatin in 1X D-PBS⁺⁺ for 1 hour at 37 °C. The gelatin solution was aspirated, and coverslips were washed with 1X D-PBS⁺⁺. The gelatin coating was crosslinked using 1% glutaraldehyde (Electron Microscope Sciences, #16220) for 30 minutes at room temperature. The glutaraldehyde was aspirated, and coverslips were incubated for 5 minutes with 1X PBS, for a total of three washes. To quench any remaining glutaraldehyde, 1 M ethanolamine (Thermo Scientific, #451762500) was added to each well and incubated for 30 minutes at room temperature. The ethanolamine was aspirated, and coverslips were again washed for 5 minutes with 1X PBS, three times. After the final wash, complete medium was added to each well, and coverslips were stored at 37 °C until cell seeding. 2.0 × 10⁵ cells per 18 mm coverslip or 1.0 × 10⁵ cells per 12 mm coverslip were seeded. When cells reached 100% confluency (approximately 48 hours after seeding), the medium was replaced and supplemented with 50 μg/mL ascorbic acid (Thermo Scientific, #352685000). Every 48 hours following, half of the medium was replaced with complete medium containing 100 μg/mL ascorbic acid.

Functional blocking of integrin β1

Sned1^KO iMEFs overexpressing SNED1-GFP were seeded and supplemented with ascorbic acid as described above. Approximately 72 hours after seeding, cells were treated with either hamster IgM (20 μg/mL; Biolegend, #401014) or anti-integrin β1 (20 μg/mL; BD Biosciences, #BDB555002). Subsequently, cell cultures were treated with antibodies every 48 hours until day 6 post-seeding, before decellularization.

Decellularization

After a specified time in culture (3 or 9 days), cell layers were decellularized. Cells were washed twice in 1X PBS, then washed three times using pre-warmed wash buffer 1 (100 mM Na₂HPO₄, 2mM MgCl₂, 2 mM EGTA, pH 9.60). Cells were then incubated with pre-warmed lysis buffer (8 mM Na₂HPO₄, 3% IGEPAL CA-360, pH 9.60) for 15 minutes at 37 °C. After adding the lysis buffer, the plate was tilted to aid cell lysis. Tilting was repeated every 5 minutes, for a total of three times. The lysis buffer was replaced for a second and then a third incubation with fresh, pre-warmed lysis buffer, for 40 minutes at 37 °C. Samples were then incubated with pre-warmed wash buffer 2 (10 mM Na₂HPO₄, 300 mM KCl, pH 7.50) containing 0.5% deoxycholate (Thermo Scientific, #J6228822) for 3 minutes, then washed three times with wash buffer 2, followed by four washes in deionized water at room temperature. 1X PBS was added to coverslips, and the samples were kept protected from light in aluminum foil at 4 °C until further processing. After each step, coverslips were visualized using a light microscope to assess cell removal.

Assembly of exogenous SNED1 by cells

The assay was performed as previously described (Vega et al., 2020). In brief, 1.0 × 10⁵ cells were seeded on sterile 12-mm coverslips in DMEM supplemented with 2 mM glutamine and 10% fibronectin-depleted FBS. The medium was replaced the next day, and cells were incubated in the presence of 10 μg/mL of human plasma fibronectin (Millipore Sigma, #FC010) or SNED1^WT-FLAG, and cultured for an additional 48 h. Samples were then fixed using ice-cold methanol:acetone (1:1) at −20 °C for 15 minutes, stained, and imaged as described below.

VCAM-1 adhesion assay

Substrate coating

Adhesion assays were performed as previously described (Humphries, 1998; Pally et al., 2025). In brief, the wells of a 96-well plate were coated murine VCAM-1 (10 μg/ml; R&D Systems, #643-VM) overnight at 4 °C. To prevent cell adhesion to plastic, wells were blocked using 10 mg/ml heat-denatured bovine serum albumin (BSA; Sigma, #A9576). Uncoated wells blocked with BSA (10 mg/ml) alone were used as a negative control.

Cell seeding and crystal violet assay

5 × 10⁵ cells/ml single-cell suspensions of Sned1^KO iMEFs (either expressing GFP alone, SNED1^WT-GFP, or SNED1^LAV-GFP) were prepared in complete medium as previously described (Pally et al., 2025). 25,000 cells were added to each well and allowed to adhere for 60 minutes on VCAM-1 substrate at 37 °C. Loosely attached or non-adherent cells were removed by washing the wells with 100 μl of 1X D-PBS⁺⁺ three times, and remaining adherent cells were fixed using 5% glutaraldehyde for 30 minutes at room temperature. Cells were stained with 0.1% crystal violet (Alfa Aesar, #B21932) in 200 mM 2-(N-morpholino) ethanesulfonic acid (MES), pH 6.0, for 60 minutes at room temperature. After washing off the excess unbound crystal violet, the dye was solubilized in 10% acetic acid. Absorbance values were measured at λ=570 nm using a Bio-Tek Synergy HT microplate reader. Cell adhesion was determined by interpolating the absorbance values from a standard curve generated by seeding cells at several dilutions (10–100%) on wells coated with 0.01% poly-L-lysine (Sigma, #P4707) and fixing them directly by adding 5% (v/v) glutaraldehyde.

Cell-ECM alignment

To assess the ability of cells to align on decellularized ECMs, 2.0 × 10⁵ cells were seeded onto decellularized ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP or SNED1^LAV-GFP after 9 days, and allowed to adhere and grow in complete medium for 48 h. Cells were fixed using 4% paraformaldehyde (PFA) (Electron Microscope Sciences, #15710), stained and imaged as described below.

Cell adhesion and cell spreading on decellularized cell-derived ECMs

Decellularized ECMs produced by Sned1^KO iMEFs overexpressing SNED1^WT-GFP or SNED1^LAV-GFP were prepared as described above. 20,000 Sned1^KO iMEFs overexpressing GFP alone were seeded onto each coverslip and allowed to attach for 30 min (adhesion assay) or 3 hours (spreading assay) at 37 °C, 5% CO₂ in a humidified chamber. Non-adherent cells were aspirated, and coverslips were washed with PBS, three times, to remove loosely attached cells. Adherent cells were fixed using 4% PFA and processed as described below. Coverslips were imaged using a Zeiss Axio Imager Z2 microscope with a 20X objective (adhesion assay) or a 40X objective (spreading assay). Cell counting was performed using ImageJ.

Cell proliferation assay

Cell proliferation was quantified using the Methyl Thiazolyl Tetrazolium (MTT) assay as per the manufacturer’s protocol (Abcam, #ab211091). 5 × 10³ cells were seeded per well (uncoated or coated with either SNED1^WT-FLAG or SNED1^LAV-FLAG) in a 96-well plate in complete medium. Alternatively, 25 × 10⁴ cells were seeded on decellularized ECMs in complete medium. Cells were supplemented with fresh complete medium every 48 h. To measure cell proliferation, the complete medium was replaced with serum-free medium containing MTT reagent at a 1:1 ratio and incubated for 30 minutes at 37 °C in a 5% CO₂ humidified incubator in the dark. The medium was aspirated and the formazan crystals were dissolved in MTT solvent at room temperature for 15 minutes on an orbital shaker. The optical density (OD) was measured at λ=590 nm using a Bio-Tek Synergy HT microplate reader. The MTT solvent alone was used as a blank.

Immunofluorescence staining

Unless stated otherwise, cells or decellularized ECMs were fixed with 4% PFA for 15 minutes at room temperature. Free aldehyde groups were quenched using 50 mM NH₄Cl for 15 min at room temperature. Following fixation, samples are rinsed three times with 1X D-PBS⁺⁺ and stained immediately or stored in 1X PBS at 4 °C for future use.

Staining of decellularized ECMs

Decellularized and fixed ECMs were stored for 96 hours at 4 °C in 1X PBS. Samples were blocked with 1% bovine serum albumin (BSA) in 1X PBS containing 0.1% Triton X-100 at room temperature for 1 hour to prevent non-specific binding. Samples were then incubated with primary antibodies (see Supplemental Table S2) in 1X PBS containing 0.05% Triton X-100 overnight at 4 °C. The following day, coverslips were washed three times with 1X D-PBS⁺⁺ containing 0.1% Triton X-100 and then incubated with secondary antibodies in 1X PBS + 0.05% Triton X-100 for 1 hour at room temperature. Coverslips were washed three times with 1X D-PBS⁺⁺ + 0.1% Triton X-100, then mounted on glass microscope slides using Fluoromount G. The slides were stored at 4 °C overnight. Coverslips were imaged using either the Zeiss Axio Imager Z2 or the Zeiss Confocal LSM 880, as indicated in the figure legends.

Staining of the actin cytoskeleton

Fixed cells were permeabilized using 0.25% Triton X-100 in 1X PBS for 10 minutes at room temperature and stained with phalloidin-AlexaFluor 647 (Invitrogen, #A22287) and 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen #D1306) for 1 hour at room temperature. Coverslips were washed three times with 1X D-PBS⁺⁺ containing 0.1% Triton X-100 for 5 minutes at room temperature. Coverslips were mounted on glass slides using Fluoromount G (Invitrogen, #00-4958-02) and allowed to dry overnight at 4 °C before imaging.

Imaging

Image acquisition

Stained samples were imaged using a Zeiss Axio Imager Z2 epifluorescence microscope or a Zeiss Confocal LSM 800 microscope, as indicated.

Image analysis

ECM thickness analysis

Z-stacks obtained via confocal microscopy were analyzed to measure the thickness of different ECM layers. The bottom-most (beginning) and top-most (end) slices where a non-noise (i.e., specific) signal was observed were recorded. Thickness was calculated by taking the total thickness of the Z-stack divided by the total number of slices to obtain the thickness of each slice. The beginning slice number was subtracted from the end slice number, and the resulting value was multiplied by the slice thickness (0.33–0.35 μm) to obtain the protein layer thickness value. These values were calculated for 3 to 5 fields per coverslip, for each protein.

3D reconstruction

The volume of each Z-stack was visualized using Fiji ImageJ. Volume Viewer plugin was used to analyze stacks, with the Z-aspect set to 10.0 in Max Projection mode with Tricubic smooth interpolation. XY, XZ, and XY snapshots were obtained for each field.

Colocalization analysis

Colocalization analysis was performed on Z-stacks using Zeiss ZEN. Thresholds were set using negative controls, and analyses between channels for each z-slice were recorded using Microsoft Excel. Line graphs using the Manders correlation coefficient were then plotted to visualize the changes across the specified portion of each Z-stack (see Leverton et al., 2026).

Area fraction density analysis

ECM density/area fraction was measured using Fiji ImageJ. 8-bit orthogonal projection (maximum intensity) images were imported and brightness and contrast were adjusted manually. Area fraction was selected in the analyze > set measurements table to obtain a staining density value for 3 to 5 fields per coverslip.

Fiber alignment analysis

The OrientationJ plugin was used to analyze F-actin alignment in cells and alignment of ECM fibers (Püspöki et al., 2016; Rezakhaniha et al., 2012). In brief, 8-bit orthogonal maximum intensity projection images were imported for measurement. The images were aligned along the dominant horizontal axis using the horizontal alignment tool in OrientationJ plugin. a 1-pixel local window was specified, and the cubic spline gradient was moved across the image to calculate structure tensors to determine local orientations. Orientation in degrees, ranging from 90° to +90° from the horizontal, was obtained as a histogram of the distribution of orientations.

Statistical analysis

All experiments were performed at least three biological replicates unless mentioned specifically in respective figure legends. Data is represented as individual experimental values, mean or mean ± standard deviation, as indicated in the respective figure legends. Unpaired, two-tailed t-tests with Welch’s correction was performed to assess statistical significance in all analyses. Graphs were generated using GraphPad Prism.

DATA AVAILABILITY

All relevant data can be found within the article and its Supplemental information. Research materials are available upon request via email to Dr. Naba (anaba@uic.edu).