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. 2002 Oct;22(19):6689–6696. doi: 10.1128/MCB.22.19.6689-6696.2002

FIG. 4.

FIG. 4.

Representative Q-PCR graphs showing the relative amounts of regions RSV LTR 1 and Luc 2 immunoprecipitated from episome pLTR-me. (A) TaqMan traces showing that the antiacetylated H3/H4 sample and the no Ab sample contain similar amounts of DNA from RSV LTR region 1. Virtually none of the RSV LTR region 1 DNA on pLTR-me is associated with acetylated H3 and H4 histones in 293/EBNA1 cells. (B) TaqMan traces showing that a considerable amount of DNA from the Luc 2 region was immunoprecipitated by antiacetylated histone H3 and H4 IgG, demonstrating that this region of pLTR-me is associated with acetylated H3 and H4 histones in 293/EBNA1 cells. The arbitrary amplification threshold is depicted as a thick horizontal line in each graph. RFU, relative fluorescence units.