TABLE 3.

Many taf1 ccr4-not double mutants are inviable^a

TAF1 allele	Viability of transformant carrying:
	wt	not1-2	not2-1	not4-1	not5-1	caf1Δ
TAF1	+	+	+	+	+	+
taf1-1	+	+	+	+	+	+
taf1-4	+	+	−	−	−	−

^aStrains carrying a disruption of the chromosomal copy of TAF1 and an episomal copy of TAF1 on a URA3 plasmid and additionally bearing the indicated ccr4-not mutation were transformed with a HIS3 plasmid carrying the indicated TAF1 allele. +, growth of transformant on FOA; −, no growth. Absence of growth on FOA indicates inviability of the double mutants. wt, wild-type allele.