FIG. 1.

Generation of MBL-A^−/− mice. (A) Maps of the genomic MBL-A locus and MBL-A targeting vector. DTA, diphtheria toxin A; H, HindIII; B, BamHI. (B) ES cell screening for homologous recombination. A 420-bp probe containing exon 1 was used for hybridization to HindIII-digested genomic DNA. The wild-type (5-kb) and targeted (3-kb) fragments are indicated. (C) Detection of MBL-A mRNA by Northern blotting. Total RNA (30 μg/lane) from the livers of thioglycolate-stimulated mice was probed with a DNA fragment containing exon 5. A β-actin probe was used as a control.