Fig. 1. Map of A. baumannii AB5075 p1AB5075 (a), Resistance Island-2 (b) and complementation plasmid pWH1266 (c). (a) Aminoglycoside genes are highlighted in orange, chloramphenicol resistance gene cmlA in pink, sulphonamide resistance gene sul1 in light green, beta-lactamase-encoding blaGES-11 in light blue and trimethoprim resistance gene dfrA7 in purple. The map was created with Proksee [35]. (b) Linear depiction of p1AB5075-encoded Resistance Island-2. (c) Schematic map of the pWH1266 plasmid used for complementation. The blaTEM-1 gene of pWH1266 was replaced by the ARG (blue) resulting from transcription of ARGs from the PblaTEM-1 promoter (grey, Pbla). All ARGs are translated using the same ribosome-binding site (orange, RBSAGGAGG). The tetA gene of pWH1266 confers resistance to tetracycline. The plasmid is not drawn to scale.
