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. 2026 Jul 24;75(7):002189. doi: 10.1099/jmm.0.002189

Fig. 3. Antibiotic disc diffusion assays comparing A. baumannii AB5075 wild-type (WT, a) and Δp1AB5075 (b) strains carrying pWH1266 or pWH1266-ARG: pWH1266-adh (c), pWH1266-aadB (d), pWH1266-aadA2 (e), pWH1266-aacA4 (f), pWH1266-strA (g) or pWH1266-strB (h). AK (amikacin 30 µg), SXT (trimethoprim–sulphamethoxazole 25 µg), TOB (tobramycin 10 µg), S (streptomycin 25 µg), CN (gentamicin 30 µg) and K (kanamycin 5 µg). MH2 agar plates containing tetracycline were lawned with wild-type A. baumannii AB5075 and Δp1AB5075 and antibiotic-containing discs were placed on the agar surface. The plates were incubated for 24 h at 37 °C.

Disc diffusion assay plates show zones of inhibition for AB5075 wild-type and delta p1AB5075 strains carrying pWH1266 or pWH1266 with ARGs including adh, aadB, aadA2, aacA4, strA, and strB against aminoglycosides and SXT.