Table 2. Average of inhibition zones of AB5075 mutants measured in mm (n=3) determined by Kirby–Bauer disc diffusion assays.
| Aminoglycoside | Tetracyclines | β-Lactam | Others | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AK 30 |
CN 30 |
K 5 |
S 25 |
TOB 10 |
TE 30 |
TGC 15 |
IPM 10 |
MEM 10 |
DOR 10 |
CFP 30 |
CAZ 30 |
FEP 30 |
ATM 30 |
TIC 75 |
OX 1 |
FOX 30 |
TZP 110 |
CIP 5 |
C 30 |
SXT 25 |
F 100 |
E 15 |
VA 30 |
|
| Wild-type | 7 | 0 | 0 | 9 | 10 | 24 | 19 | 12 | 9 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 22 | 0 |
| Δp1AB5075 | 21 | 20 | 17 | 16 | 18 | 22 | 17 | 13 | 10 | 12 | 0 | 19 | 0 | 7 | 0 | 0 | 0 | 0 | 0 | 7 | 25 | 0 | 20 | 0 |
| Wild-type pWH1266 | 9.5 | 10 | 0 | 12 | 11 | 10 | 18 | 12 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 21 | 0 |
| Δp1AB5075 pWH1266 | 24 | 21 | 19 | 15 | 18 | 10 | 19 | 12 | 9 | 12 | 0 | 17 | 18 | 8 | 0 | 0 | 0 | 0 | 0 | 7 | 26 | 0 | 19 | 0 |
| Δp1AB5075 pWH1266-aadB | 21 | 0 | 0 | 15 | 0 | 10 | 15 | 14 | 10 | 13 | 0 | 19 | 15 | 10 | 0 | 0 | 0 | 0 | 0 | 10 | 27 | 0 | 20 | 0 |
| Δp1AB5075 pWH1266-adh | 0 | 23 | 0 | 16 | 19 | 10 | 16 | 12 | 10 | 12 | 0 | 20 | 17 | 9 | 0 | 0 | 0 | 0 | 0 | 9 | 28 | 8 | 20 | 0 |
| Δp1AB5075 pWH1266-aacA4 | 21 | 20 | 8 | 16 | 14 | 9 | 18 | 14 | 14 | 14 | 0 | 22 | 19 | 11 | 0 | 0 | 0 | 0 | 0 | 12 | 25 | 7 | 21 | 0 |
| Δp1AB5075 pWH1266-aadA2 | 27 | 22 | 19 | 0 | 18 | 9 | 15 | 12 | 10 | 11 | 0 | 18 | 17 | 10 | 0 | 0 | 0 | 0 | 0 | 8 | 29 | 7 | 19 | 0 |
| Δp1AB5075 pWH1266- strA | 27 | 25 | 18 | 0 | 17 | 11 | 18 | 14 | 10 | 12 | 0 | 18 | 15 | 10 | 0 | 0 | 0 | 0 | 0 | 10 | 30 | 8 | 20 | 0 |
| Δp1AB5075 pWH1266- strB | 24 | 23 | 17 | 17 | 18 | 10 | 15 | 12 | 10 | 11 | 0 | 20 | 19 | 12 | 0 | 0 | 0 | 0 | 0 | 11 | 28 | 8 | 20 | 0 |
| Δp1AB5075 pWH1266-blaGES-11 | 23 | 23 | 26 | 12 | 21 | 0 | 17 | 13 | 10 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 30 | 8 | 21 | 0 |
AK, amikacin; ATM, aztreonam; C, chloramphenicol; CAZ, ceftazidime; CFP, cefoperazone; CIP, ciprofloxacin; CN, gentamicin; DOR, doripenem; E, erythromycin; IPM, imipenem; K, kanamycin; MEM, meropenem; OX, oxacillin; S, streptomycin; SXT, trimethoprim/sulphamethoxazole; TE, tetracycline; TGC, tigecycline; TIC, ticarcillin; TOB, tobramycin; TZP, piperacillin/tazobactam; VA, vancomycin.