Table 4. MIC of antibiotics determined by broth microdilution assay (n=4).
Values are in μg ml−1. CN (gentamicin), K (kanamycin), AK (amikacin), S (streptomycin), TOB (tobramycin), FEP (cefepime), CTX (cefotaxime), ATM (aztreonam), IPM (imipenem), MEM (meropenem), DOR (doripenem). nd (not determined). S (susceptible), I (intermediate), R (resistant).
| Strain | CN | K | AK | S | TOB | FEP | CTX | ATM | IPM | MEM | DOR |
|---|---|---|---|---|---|---|---|---|---|---|---|
| WT pWH1266 | >256 (R) | > 2048 | 128 (R) | 128 | 16 (R) | >256 (R) | 2048 (R) | >256 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266 | 8 (I) | <4 | <4 (S) | 16 | <0.5 (S) | 32 (R) | 32 (I) | 64 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-aadB | > 256 (R) | 512 | <4 (S) | 64 | 128 (R) | 32 (R) | 32 (I) | 32 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-adh | 8 (I) | >2048 | 512 (R) | 32 | <0.5 (S) | 32 (R) | 32 (I) | 64 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-aadA2 | 8 (I) | <4 | <4 (S) | 1024 | <0.5 (S) | 32 (R) | 32 (I) | 32 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-aacA4 | 32 (R) | 128 | 8 (S) | 32 | 16 (R) | 32 (R) | 32 (I) | 32 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-strA | 8 (I) | <4 | <4 (S) | 1024 | <0.5 (S) | 32 (R) | 32 (I) | 32 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-strB | 8 (I) | <4 | <4 (S) | 32 | <0.5 (S) | 16 (I) | 32 (I) | 32 | 16 (R) | 16 (R) | 16 |
| Δp1AB5075 pWH1266-blaGES-11 | nd | nd | nd | nd | nd | 256 (R) | 1024 (R) | >256 | 16 (R) | 16 (R) | 16 |