FIG. 2.

T-cell-independent type II antigen capturing in wild-type and BOB.1/OBF.1^−/− mice. (A) Splenocytes from TNP-Ficoll-immunized wild-type and BOB.1/OBF.1^−/− mice were stained with a combination of anti-B220, anti-CD21, anti-CD23, and anti-TNP antibodies. MZ B cells (B220^pos CD21^high CD23^low) were gated, and histograms presenting anti-TNP staining of immunized and unimmunized animals are shown. The data shown are representative of three sets of experiments. (B) Spleen sections from TNP-Ficoll-immunized animals were stained with biotin-labeled anti-TNP (red) and FITC-labeled anti-MOMA-1 (green) antibodies. Sections were mounted with Mowiol containing the DNA-specific fluorescent dye DAPI (blue), which stained all nucleated cells. Magnification, ×400.