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Lack of MZ B cells in BOB.1/OBF.1-deficient mice is due to an intrinsic defect in the B-cell lineage. (A) Wild-type and BOB.1/OBF.1−/− bone marrow cells were injected into μMT mice. Control mice were injected with PBS. After 32 days of repopulation, spleens were analyzed for the presence of CD21high CD23low (MZ) B cells. (B) One part of the spleen was used for histological examinations. Sections were stained with anti-IgM-PE and anti-MOMA-1-FITC antibodies.
