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. 2002 Dec;22(23):8320–8331. doi: 10.1128/MCB.22.23.8320-8331.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 5. — Expression of BOB.1/OBF.1 limited to bone marrow does not rescue MZ B-cell development. Mice expressing a tetracycline-regulated transactivator (tTA) under the control of the intronic μ heavy-chain enhancer (μE) and a minimal promoter and BOB.1/OBF.1 under the control of a bidirectional tetracycline operator sequence (tetO) were crossed with BOB.1/OBF.1^−/− or BOB.1/OBF.1^+/− mice in order to obtain expression of the BOB.1/OBF.1 transgene on a BOB.1/OBF.1^−/− background. (A) Splenocytes from double-transgenic (dtg) BOB.1/OBF.1^+/−, single-transgenic (stg) BOB.1/OBF.1^−/−, and double-transgenic BOB.1/OBF.1^−/− animals were stained with anti-B220, anti-CD21, and anti-CD23 antibodies. Representative CD21/CD23 stainings of B220-positive cells and mean percentages of MZ B-cell numbers are shown. (B) Splenic cryosections from the same mice were stained with anti-IgM-PE (red) and anti-MOMA-1-FITC (green) antibodies and viewed at a magnification of ×400.