FIG. 3.

(A) Bisulfite genomic sequencing of the MT-I promoter in lymphosarcoma cells treated with different inhibitors. Genomic DNA isolated from cells was treated with sodium metabisulfite reagent to convert unmethylated cytosines to uracils. The MT-I promoter was then amplified by nested PCR with gene-specific primers and sequenced directly with an internal primer specific for the upper strand. The closed, hatched, and open circles indicate completely methylated, partially methylated, and unmethylated cytosines, respectively. (B) Schematic representation of the methylation status of 18 CpG dinucleotides located within −190 bp of the MT-I transcription start site in cells treated with the inhibitors.