TABLE 1.
Ability of Q34 cells to form anchorage-independent coloniesa
| Cell strain and/or virus | % Cells forming agar colonies |
|---|---|
| Q34 + pBABE | 4 |
| Q34 + RAS | 29 |
| Q34 + hTERT + pBABE | 1 |
| Q34 + hTERT + RAS | 26 |
| Hs68 + pBABE | 1 |
| Hs68 + RAS | 0 |
| Hs68 + hTERT + pBABE | 0 |
| Hs68 + hTERT + RAS | 0 |
a
Cells were suspended in medium containing 0.2% agarose, and colonies were counted after 21 days. Multiple fields of >100 cells were observed microscopically, and the number of multicellular colonies was estimated as a percentage of the total number of cells visualized.