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. 2002 Dec;22(23):8332–8341. doi: 10.1128/MCB.22.23.8332-8341.2002

FIG. 1.

FIG. 1.

Western blotting with specific antibodies to GFP of PC12 Tet-On (control) and stable transfected PC12/FMR7 cells with (+) and without (−) DOX induction. PC12/FMR7 cells show the presence of a band of approximately 110 kDa after DOX induction with the anti-GFP antibody, which is the expected size for the full-length FMRP-GFP fusion protein (80 plus 27 kDa). In contrast, PC12/FMR7 cells without DOX induction are devoid of the 110-kDa band, representing the absence of nonspecific induction (leakage). In PC12 Tet-On cells no detection of the 110-kDa band was observed. Numbers at left show molecular masses in kilodaltons.