FIG. 8.

yng2 mutants are able to perform SSA repair. (A) Schematic of the leu2 direct repeat cassette used to measure an HO-induced recombination event measured by loss of the URA3 gene. (B) Wild-type (YNG2) cells and yng2 mutant cells containing this cassette were transformed with a TRP1 plasmid containing a galactose-inducible HO endonuclease gene. Cells were taken at the indicated times post-galactose addition, and the number of cells that had undergone a recombination event and lost the URA3 gene was determined. YNG2 and yng2 mutant cells display similar levels of SSA. Footnote a, approximately 200 to 900 colonies were scored for each time point. The percentage of deletion events is a measure of the total number of colonies that have lost Ura prototrophy relative to the total number of cells plated that were Trp+.