TABLE 1.
S. cerevisiae strains used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| 1783 | MATaa | I. Herskowitz |
| 1788 | MATa/MATαa | I. Herskowitz |
| DL376 | MATapkc1Δ::LEU2a | 29 |
| DL1248 | MATα stt1-1 pRS315[PKC1 ADE3]b | This study |
| DL2312 | MATabck1Δ::HIS4a | This study |
| DL2315 | MATa/MATα bck1Δ::HIS4/BCK1a | This study |
| DL2745 | MATabck1Δ::HIS4 htl1Δ::TRP1a | This study |
| DL2749 | MATahtl1Δ::TRP1a | This study |
| DL2751 | MATα htl1Δ::TRP1a | This study |
| DL2754 | MATa/MATα htl1Δ::TRP1/htl1Δ::TRP1a | This study |
| DL2822 | MATα “pet18” isolate of DL1248#1b | This study |
| DL2823 | MATα “pet18” isolate of DL1248#2b | This study |
| MCY3890 | MATaswh3-ts16 (rsc8ts)c | 44 |
| YBC628 | MATarsc3Δ::HIS3 pRS316[RSC3]d | 1 |
| YBC840 | MATarsc3Δ::HIS3 pRS315[rsc3-1]d | 1 |
| YBC842 | MATarsc3Δ::HIS3 pRS315[rsc3-2]d | 1 |
| YBC843 | MATarsc3Δ::HIS3 pRS315[RSC3]d | 1 |
| YBC906 | MATarsc3Δ::HIS3 pRS315[rsc3-3]d | 1 |
| YBC928 | MATahis3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 trp1Δ63 pep4Δ::Kanmx | This study |
| YBC1334 | MATarsc3Δ::HIS3 htl1Δ::TRP1 pRS316[HTL1] pRS315[RSC3]d | This study |
| YBC1336 | MATarsc3Δ::HIS3 htl1Δ::TRP1 pRS316[HTL1] pRS315[rsc3-1]d | This study |
| YBC1338 | MATarsc3Δ::HIS3 htl1Δ::TRP1 pRS316[HTL1] pRS315[rsc3-2]d | This study |
| YBC1340 | MATarsc3Δ::HIS3 htl1Δ::TRP1 pRS316[HTL1] pRS315[rsc3-3]d | This study |
a
EG123 background (leu2-3,112 trp1-1 ura3-52 his4 can1).
b
ade2 ade3 ura3 leu2 trp1 his3.
c
W303-1A background (trp1-1 leu2-3,112 his3-11,15 ura3-1 ade2-1 can1-100).
d
his3Δ200 leu2Δ1 lys2-128Δ trp1Δ63 ura3-52.