FIG. 7.

mtGPAT^−/− mice have altered liver PE fatty acid composition. PE from mtGPAT^+/+ and mtGPAT^−/− mice (n = 6 per genotype) was separated from other liver phospholipids by solid-phase column chromatography. After methyl esterification, total PE fatty acids were quantified by GC. A portion of the PE fraction was digested with PLA₂ and then subjected to acid hydrolysis. (A) Total fatty acids. (B) sn-1 fatty acids. (C) sn-1 fatty acid classes (Sat, saturated; Mono, monounsaturated; Poly, polyunsaturated). (D) sn-2 fatty acids. (E) sn-2 fatty acid classes. Only the major fatty acids are presented. a, P < 0.05; b, P < 0.005; c, P < 0.001.