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The sub2-201 mutation is synthetically lethal with deletion of any one of the HPR1, THO2, and MFT1 genes of the THO complex. Δsub2, Δsub2/Δhpr1, Δsub2/Δtho2, and Δsub2/Δmft1 cells, expressing Sub2-201p from a TRP1-marked plasmid and Sub2p from a URA3-marked plasmid, were grown at 24°C on medium containing 5-FOA (to rid the cells of Sub2p) or on URA dropout plates (CSM-URA), as indicated.
