Skip to main content
. 2026 Jul 27;16:23328. doi: 10.1038/s41598-026-61694-1

Table 3.

Summary of water temperatures by breeding site characteristics across the four study locations.

Ecological zone and study location Terai Chitwan (urban) ≤ 200 m Mid-Hills Kathmandu (urban) 1300-1400 m Mid-Hills Bhaktapur (rural) 1300-1400 m Mountain Lalitpur (rural) > 1600 m Overall
Mean temperatures (temp) Mean temp (SE) No. of sites Mean temp (SE) No. of sites Mean temp (SE) No. of sites Mean temp (SE) No. Of sites Mean temp (SE) No. of sites
Breeding site characteristics
Material
Plastic 27.8 (0.5) 25 23.5 (0.4) 40 23.6 (0.8) 15 24.0 (0.7) 28 24.6 (0.3)a 108
Metal/tin 28.3 (3.0) 2 23.9 (2.0) 2 24.7 (0.9) 11 23.6 (1.4) 8 24.6 (0.7) 23
Rubber tyre 25.2 (1.1) 7 19.9 (0.3) 4 23.4 (0.6) 3 18.4 (0.1) 3 22.4 (0.8) 17
Other 25.5 (1.8) 2 23.4 (0.4) 4 21.8 (1.0) 9 22.8 (1.0) 8 22.7 (0.6) 23
Anova p-values  p = 0.168 p = 0.055 p = 0.183 p = 0.072 p = 0.012*
Colour
Dark 27.1 (0.7) 23 23.5 (0.5) 34 22.0 (0.6) 15 23.3 (0.6) 33 24.0 (0.3) 105
Light 27.3 (0.7) 13 22.7 (0.6) 16 24.4 (0.6) 23 23.4 (1.2) 14 24.4 (0.4) 66
Anova p-value p= 0.852 p = 0.303 p = 0.014* p = 0.905 p = 0.499
Size
Small 27.3 (0.7) 20 22.5 (0.5) 10 24.4 (0.5) 18 24.6 (1.2) 13 25.1 (0.5)c 61
Medium 26.9 (0.7) 15 22.5 (0.5) 21 22.8 (0.6) 15 23.0 (0.9) 17 23.7 (0.4) 68
Large 29.1 (—) 1 24.3 (0.7)b 19 22.2 (1.5) 5 22.8 (0.7) 17 23.6 (0.5) 42
Anova p-value p = 0.733 p = 0.042* p = 0.163 p = 0.332 p = 0.023*
Debris
Debris ≤ 50% 27.1 (0.6) 25 23.5 (0.4) 38 24.0 (0.6) 24 23.4 (0.7) 31 24.4 (0.3) 118
Debris > 50% 27.3 (0.8) 11 22.4 12 22.6 (0.7) 14 23.2 (0.8) 16 23.7 (0.4) 53
Anova p-value p = 0.920 p = 0.193 p = 0.146 p = 0.864 p = 0.245
Sunlight
Sunlight ≤ 50% 25.7 (0.8) 14 22.1 (0.3) 29 23.6 (0.3) 15 21.6 (0.6) 6 23.2 (0.3) 64
Sunlight > 50% 28.1 (0.6) 22 24.7 (0.6) 21 23.4 (0.8) 23 23.6 (0.6) 41 24.7 (0.4) 107
Anova p = 0.017* p < 0.001** p = 0.848 p = 0.202 p = 0.005*

* Significant at the 0.05 level.

** Significant at the 0.01 level.

Tukey HSD results.

a. Plastic breeding site water temperatures significantly higher than rubber (p = 0.046) and other (p = 0.05).

b. Large breeding site water temperatures not significantly higher than medium sized breeding sites (p = 0.055).

c. Small breeding site water temperatures significantly higher than medium (p = 0.029) and large (p = 0.047) breeding sites.