B/B, B/P, PB/B, PB/P	Hstx2/Prdm9 allelic-combination shorthand (B = B6 allele; P = PWD allele)
B6	C57BL/6J inbred strain
BC1	first backcross generation
BCA	bicinchoninic acid assay
BH	Benjamini–Hochberg
BSA	bovine serum albumin
CCD	charge-coupled device
CCP	Czech Centre for Phenogenomics
CRISPR/Cas9	clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9
DAPI	4′,6-diamidino-2-phenylindole
DSB	DNA double-strand break
ECL	enhanced chemiluminescence
F1	first filial generation
FDR	false discovery rate
HRP	horseradish peroxidase
IMG CAS	Institute of Molecular Genetics of the Czech Academy of Sciences
IQR	interquartile range
JT	Jonckheere–Terpstra
KO	knockout
LMM	linear mixed model
MiniMUGA	Miniature Mouse Universal Genotyping Array
MMR	DNA mismatch repair
OR	odds ratio
PBS	phosphate-buffered saline
PCR	polymerase chain reaction
PVDF	polyvinylidene difluoride
PWD	PWD/Ph inbred strain
RIPA	radioimmunoprecipitation assay buffer
rTW	relative testes weight
SC	sperm count
SDS-PAGE	sodium dodecyl sulphate–polyacrylamide gel electrophoresis
sgRNA	single-guide RNA
SNP	single-nucleotide polymorphism
SPF	specific pathogen-free
SSLP	simple sequence length polymorphism
wt	wild type