Figure 3: PCSK inhibition accelerates the normal temporal pattern of COC gene expression and results in dysregulation of pathways related to the ECM, cell adhesion, cell migration, and metabolism.

A) Schematic showing methodology for collecting COCs for bulk sequencing. IVM = in vitro maturation. B) Heatmap showing relationships between bulk sequenced samples with unbiased clustering. Red and blue indicate high and low correlations between samples, respectively. C) Volcano plots showing number of up- and down-regulated genes in PCI-treated COCs relative to control COCs at each timepoint. D) Dot plot showing pathways downregulated with PCI treatment at 8 h. E) Heatmap of top genes associated with ECM organization and cell adhesion that are downregulated by PCI treatment. F) Dot plot showing pathways upregulated with PCI treatment in 8 h COCs. G) Heatmap of top genes associated with cell migration that are upregulated by PCI treatment.