Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-mouse Furin | Thermo-Fisher Scientific | Cat#PA1-062; RRID: AB_2105077 |
| Rabbit polyclonal anti-mouse PCSK5 | Proteintech | Cat#16470-1-AP; RRID: AB_2283381 |
| Chemicals, peptides, and recombinant proteins | ||
| Proprotein convertase inhibitor | Sigma-Aldrich | Cat#537076 |
| Furin inhibitor II | Sigma-Aldrich | Cat#SCP0148 |
| Mouse GDF9 | R&D Systems | Cat#739-G9-010 |
| Biotinylated hyaluronic acid binding protein | Sigma-Aldrich | Cat#385911 |
| Critical commercial assays | ||
| Live/dead™ Viability/Cytotoxicity Kit, for mammalian cells | Invitrogen | Cat#L3224 |
| RNAscope 2.5 HD Red Assay | Advanced Cell Diagnostics 27 | Cat#322360 |
| Vectastain Elite ABC Kit | Vector Laboratories | Cat#PK-6101 |
| DAB Peroxidase (HRP) Substrate Kit | Vector Laboratories | Cat#SK-4100 |
| RNeasy Micro Kit | Qiagen | Cat#74004 |
| TSA (Tyramide Signal Amplification) plus fluorescein kit | Akoya Biosciences | Cat# NEL741001KT |
| Deposited data | ||
| Gene annotation file for mm39 | Ensembl | http://useast.ensembl.org/index.html |
| Single cell sequencing of mouse ovary during ovulation | Huang et al. | GEO: GSE294534 |
| Raw and analyzed bulk RNAseq data | This paper | GEO: GSE331136 |
| Raw and analyzed proteomics data | This paper | ID: MSV000102085 |
| Experimental models: Organisms/strains | ||
| Mouse: ICR CD-1© | Envigo | 030 |
| Oligonucleotides | ||
| RNAscope probe for Pcsk3 | ACD | Cat#864041 |
| RNAscope probe for Pcsk5a | ACD | Cat#1241481-C1 |
| RNAscope probe for Pcsk5b | ACD | Cat#1241471-C1 |
| RNAscope probe for Pcsk6 | ACD | Cat#593361 |
| RNAscope probe for Ppib | ACD | Cat#313911 |
| RNAscope probe for Dapb | ACD | Cat#310043 |
| Software and algorithms | ||
| Fiji | Schneider et al. | https://imagej.nih.gov/ij/ |
| cutadapt | Martin | https://github.com/marcelm/cutadapt |
| STAR | Dobin et al. | https://github.com/alexdobin/STAR |
| htseq-count | Anders et al. | https://github.com/simon-anders/htseq |
| PCAtools | Blighe et al. | https://github.com/kevinblighe/PCAtools |
| ComplexHeatmaps | Gu et al. | https://github.com/jokergoo/ComplexHeatmap |
| pheatmap | Kolde | https://github.com/raivokolde/pheatmap |
| DESeq2 | Love et al. | https://github.com/thelovelab/DESeq2 |
| Enrichr | Chen et al.; Kuleshov et al.; Xie et al. | https://maayanlab.cloud/Enrichr/ |