Figure 5. Wnt signaling is required for proper canal form and function.

A-B. Representative image of an OV in a tyrosinase CRISPant(control) (A) and a wls CRISPant (B). C. Quantification of pillar aspect ratio of tyrosinase (control, black) CRISPants and wls (grey) CRISPants by pillar type indicated. D. Quantification of pillar angle of tyrosinase (control, black) CRISPants and wls (grey) CRISPants by pillar type indicated. Coefficient of variation (cv) indicated below each dataset, calculated as the standard deviation from the mean divided by the mean. E-F. Representative images of an OV in a wls CRISPant before (E) and 4 hpt (F) with DMSO by soaking. G-H. Representative images of an OV in a wls CRISPant before (G) and 4 hpt (H) with Forskolin by soaking. I-J. Quantification of OV lumen volume (pL) in wls CRISPants before and 4 hpt with either DMSO (I) or Forskolin (J). P-values as labeled (two-tailed paired t-test). K-L. Two representative minimum projections of membrane-mNeonGreen (NG control) 5 dpf larvae swimming in the MCAM channel for 10 minutes. M-N. Two representative minimum projections of uninflated swimbladder membrane-mNeonGreen (swimbladder control) 5 dpf larvae swimming in the MCAM channel for 10 minutes. O-P. Two representative minimum projections of wls^−/−; membrane-mNeonGreen (wls mutant) 5 dpf larvae swimming in the MCAM channel for 10 minutes. Q. Kymograph of average swimming speed in 0.2-second time bins over time for every NG control, swimbladder control, and wls mutant larva assayed. Each row represents one larva. R-S. Two representative minimum projections of membrane-mNeonGreen (NG control) 6 dpf larvae swimming in the startle response chamber for 308 milliseconds in response to a backwards water current startle trigger. T-U. Two representative minimum projections of uninflated swimbladder membrane-mNeonGreen (swimbladder control) 6 dpf larvae swimming in the startle response chamber for 308 milliseconds in response to a backwards water current startle trigger. V-W. Two representative minimum projections of wls^−/−; membrane-mNeonGreen (wls mutant) 6 dpf larvae swimming in the startle response chamber for 308 milliseconds in response to a backwards water current startle trigger. X. Quantification of swimming path straightness in response to a backwards water current startle trigger in NG controls, swimbladder controls, or wls mutants. Path straightness defined as the net displacement in 308 milliseconds divided by the total path length traversed over 308 milliseconds. P-values as labeled (Kruskal-Wallis test). “n” denotes the number of embryos. Scale bar: 50 μm. Data are mean±s.d. P-values as labeled (unpaired, two-tailed Student’s t-test, unless otherwise specified). Scale bars in K-P and R-W are all 2 mm.