Figure 2.

Timelapse live imaging and single cell tracking for motility analysis of HER2 Crainbow cell lines. A, Schematic of experimental conditions and cell tracking approach. B, Snapshots of a representative well seeded on tissue culture plastic at 10,000 initial cell density with single cell tracks overlaid. C, Region of interest from “B”. D, Diagram of spatial correlation analysis using a 50μm neighborhood. E, Principal component analysis (PCA) of motility and spatial crowding metrics across all conditions and colored by genotype. F&G, PCA of motility and spatial crowding metrics across all experiments colored by initial cell seeding density (F) and surface coating (G).