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. 2006 Jan 18;34(2):451–461. doi: 10.1093/nar/gkj455

Figure 3.

Figure 3

(A) QRT-PCR assay performed on RNA isolated from K562 cells transfected with 2′F-ANA and PS-DNA molecules. Data are presented as a function of c-MYB mRNA copies relative to GAPDH RNA. Mock (control) cells were subjected to nucleoporation, but in the absence of ODN. MOH10 antisense is designed to target c-MYB mRNA region slightly shifted toward the 3′ end compared with the MOH1 and MOH2 sequences. (B) c-Myb protein western blot analysis performed on cell lysate obtained from K562 cells transfected with 2′F-ANA and PS-DNA molecules. Real-time PCR and western blot were performed 24 h post nucleofection.