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. 2006 Jan 18;34(2):e10. doi: 10.1093/nar/gnj013

Figure 1.

Figure 1

A gene conversion substrate using a non-Ig exogenous gene. (A) Gene conversion events in the Ig loci result in the introduction of pseudo-V gene segments into the expressed V genes. (B) The structure of G/B construct, a gene conversion substrate, and the strategy for its targeting to the IgL locus. C λ, the constant region of λL chain; VJ λ, the rearranged V region of λ L chain; Ba, BamHI; pA, the polyadenylation signal; CMVp, the cytomegalovirus promoter; SV40p, the simian virus 40 promoter. (C) Color shift (from blue to green) of BFP by C199T mutation caused by the gene conversion of G/B construct. (D) Confirmation of targeted integration of G/B or 0/B construct in the IgL locus. PCR using a primer pair [see arrowheads in (B)] gave the expected length of PCR products as illustrated in (B).