FIG. 6.

Inactive ARF deletion mutants, D6-10 and D21-25, bind to Mdm2 in vivo. U2OS cells were transfected with expression constructs encoding wild-type Mdm2 plus empty vector, wild-type ARF, or the indicated ARF mutants. Complexes between ARF and Mdm2 were identified by immunoprecipitation with antibodies to Mdm2 (M) and ARF (A), followed by Western blotting with both antibodies, as indicated to the right of each blot. The inactive ARF mutants which lack Mdm2 binding capability (D1-62 and D1-14;D26-37) are highlighted by asterisks.