FIG. 4.

CD4⁺ memory phenotype T cells from CaMKIV^−/− mice display impaired cytokine gene induction. CD4⁺ memory phenotype (A) and naive (B) T cells from wild-type and CaMKIV^−/− mice were stimulated with plate-bound CD3 and CD28 antibodies for 2 days. Total RNA was then isolated, and IL-2, IFN-γ, and IL-4 gene induction was measured by competitive RT-PCR as described in Materials and Methods. The numbers above the lanes indicate the picomoles of competitor construct included in the PCR. For each of the four genes examined, the PCR product arising from the competitor construct (upper arrow) runs higher in the gel compared to that from the endogenous gene (lower arrow). The results of one representative experiment (n = 4) are shown.