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Expression and activation of RSK2 in CD4+ T cells. Naive (N) and memory phenotype (M) CD4+ T cells from wild-type and CaMKIV−/− mice were probed for RSK2 and for phospho-CREB by Western blot after stimulation with PMA (10 ng/ml) and ionomycin (1 μM) (PI) for 10 min. Activated RSK2 appears as a slower-migrating band.
