FIG. 7.
In vivo suppression of the τ131-ΔTPR2 mutation and other ΔTPR mutations by overexpression of TFIIIB components. Multicopy suppression of τ131-ΔTPR2 by TFIIIB components. Stationary-phase cultures of mutant cells harboring the different multicopy plasmids indicated were diluted 10−1-, 10−2-, 10−3-, and 10−4-fold in water and spotted (5 μl) on solid yeast extract-peptone-dextrose medium and grown for three days. The different genes harbored on the high-copy-number vector pFL44L are as follows: pLR30, Brf1; pL1, TBP; pJR38, B”.