FIG. 4.

IRS-2 is required for maximal Th2 cytokine production. (A) FACS-purified CD4⁺/CD62L-high T cells were cultured under Th1 or Th2 skewing conditions for 1 week. Cytokine production from PMA-ionomycin-stimulated cells was assessed by intracellular cytokine staining. The results are representative of four independent experiments. (B) FACS-purified CD4⁺/Mel14-high T cells were cultured under Th1 (lanes 1) or Th2 (lanes 2) skewing conditions for 1 week and restimulated with anti-CD3 for 24 h. RNA was prepared from the cultured cells, and GATA-3 expression was determined by Northern blot analysis. The blot was stripped and reprobed for γ-actin.