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. 2002 Jan;22(1):332–342. doi: 10.1128/MCB.22.1.332-342.2002

FIG. 2.

FIG. 2.

Tankyrase 1 is localized to telomeres and complexed to TRF1 in vivo. (A) Colocalization of endogenous tankyrase 1 and TRF1 at telomeres. Indirect immunofluorescence analysis of swollen-formaldehyde fixed metaphase spreads from HeLaI.2.11 cells stained with anti-tankyrase 1 antibody 609 (green) and anti-TRF1 (red). Merge represents superimposition of the red and green images. DAPI staining of DNA is shown in blue. Scale bar, 5 μm. (B) Coimmunoprecipitation of an endogenous tankyrase 1-TRF1 complex from HelaI.2.11 cells. Cell extracts were immunoprecipitated (IP) with anti-tankyrase 1 antibody 376 (lanes 1 and 4), normal rabbit IgG (NR) (lanes 2 and 5), or anti-TRF1 antibody 415 (lanes 3 and 6). Samples were processed as described in Materials and Methods, suspended in Laemmli buffer, and boiled (left panel) or not boiled (right panel). Reaction products were fractionated by SDS-PAGE and subjected to Western blot (wb) analysis with anti-tankyrase 1 antibody 376 (left panel) or anti-TRF1 antibody 415 (right panel).