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. 2002 Jan;22(1):332–342. doi: 10.1128/MCB.22.1.332-342.2002

FIG. 8.

FIG. 8.

Overexpression of tankyrase 2 in the nucleus releases TRF1, but not TRF2, from telomeres. (A) Schematic diagram of the tankyrase 2 expression construct. M, Myc epitope tag; N, SV40 NLS. (B) Indirect immunofluorescence of HelaI.2.11 cells transiently transfected with MN-tankyrase 2, formaldehyde fixed, and stained with anti-myc antibody (green) (B and C) and anti-TRF1 antibody 415 (red) (B′) or anti-TRF2 antibody (red) (C′). DAPI staining of DNA is shown in blue. Scale bar, 5 μm.

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