Figure 3.

DNMT3L associates with HDAC activity. Seven different GST–DNMT3L fusion proteins were used to pull-down the HDAC activity from THP-1 monocyte total protein lysates. α-HDAC1 and α-GAL4 antibodies were used as positive and negative controls, respectively. Deacetylase activity was detected as radioactivity released from ³H-labelled acetylated histone peptide substrate. To show that the repressional effect was mediated through HDAC1, 100 mM sodium butyrate (NaBu) was added to the reactions with PHD finger-containing constructs to inhibit the deacetylation reaction.