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DNMT3L has a functional NLS. (A) DNMT3L has a functional NLS, RRRK, at amino acids 156–159. DNMT3L amino acids 142–173 were cloned into GFP vector pEGFP-C3 and transiently transfected into Cos-7 cells. (Upper) GFP fluorescence; (lower) DAPI staining of the same cell. (B) Full-length GFP–DNMT3L and (C) GFP vector alone are distributed in both the cytoplasm and nucleus.
