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Dose-dependent differential loss of Ser5-P RNAP II or non-phosphorylated RNAP II. ChIP experiment. Chromatin extracts were prepared from rad14 cells 30 min after irradiation with indicated UV doses. RNAP II was pulled down using H14 antibody (S5-P) or 8WG16 antibody (CTD). Top bands in each gel correspond to the promoter region or coding sequence of genes indicated flanking the gel. Lower bands are amplified from inactive HML locus.
