Figure 4.
Rad3 promoter localization is not altered upon UV irradiation. ChIP experiment. Triple HA-tagged Rad3 was immunoprecipitated from indicated strains using 12CA5 antibodies. (A) Rad3 localizes to the ACT1 promoter. Chromatin extracts were prepared from cells either unirradiated or from cells irradiated with 70 J/m2 10 min after irradiation. DNA was amplified from precipitates using primers corresponding to the promoter and CDS of the ACT1. (B) Rad3 localization is not altered in the absence of NER and Spt4 after a high UV dose. Rad3 was immunoprecipitated from chromatin extracts prepared from indicated strain either unirradiated or irradiated with 140 J/m2 after indicated time points (minutes). Promoter and coding sequences were amplified from the GAL7 gene.