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. 2002 Aug 15;30(16):3609–3614. doi: 10.1093/nar/gkf476

Figure 3.

Figure 3

Figure 3

The determination of the levels of target genes expression. (A) The levels of expression of target genes in cells that expressed poly(A)-connected (A60) or unconnected Bik-ribozyme, or CAD-ribozyme. Bik mRNA and CAD mRNA were detected by RT–PCR with primers specific for the respective genes (see Materials and Methods). CBP is an endogenous control. The levels of expression of all target genes that were identified using the gene discovery system in cells that expressed a poly(A)-connected ribozyme. WT, wild-type. (B) Target mRNAs were detected by RT–PCR with up primers specific for the respective genes and oligo dT down primer. UG, unknown gene.